Difference between revisions of "SdhA"

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m (Reverted edits by 134.76.70.252 (talk) to last revision by Jstuelk)
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= This gene is a member of the following [[regulons]] =
 
= This gene is a member of the following [[regulons]] =
 
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{{SubtiWiki regulon|[[FsrA regulon]]}}
  
 
=The gene=
 
=The gene=
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* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2495271 PubMed]
 
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2495271 PubMed]
  
* '''Regulation:''' constitutive  
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* '''Regulation:'''  
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** constitutive  
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** part of the iron sparing response ([[FsrA]]) {{PubMed|22389480}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
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** [[FsrA]]: translation inhibition {{PubMed|22389480}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,,17726680 18763711 17726680 16493705 3910107 6799760 12560550  </pubmed>
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<pubmed>2987185,1707123,6401289,6406223,1324713,3036777,2495271,3027051,22389480,17726680 18763711 17726680 16493705 3910107 6799760 12560550  </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 18:07, 14 March 2012

  • Description: succinate dehydrogenase (flavoprotein subunit)

Gene name sdhA
Synonyms citF
Essential no
Product succinate dehydrogenase (flavoprotein subunit)
Function TCA cycle
Interactions involving this protein in SubtInteract: SdhA
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 65 kDa, 5.714
Gene length, protein length 1758 bp, 586 aa
Immediate neighbours sdhB, sdhC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SdhA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

carbon core metabolism, membrane proteins, phosphoproteins

This gene is a member of the following regulons

FsrA regulon

The gene

Basic information

  • Locus tag: BSU28440

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Succinate + acceptor = fumarate + reduced acceptor (according to Swiss-Prot)
  • Protein family: FRD/SDH subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Cofactor(s): Fe
  • Effectors of protein activity:
    • Inhibited by 2-(n-Heptyl)-4-hydroxy-quinoline N-oxide PubMed
    • Activated by Cytochrome b558 PubMed

Database entries

  • Structure: 1NEK (E. coli)
  • KEGG entry: [3]
  • E.C. number: 1.3.99.1

Additional information

  • This enzyme is a membrane-bound trimer PubMed PubMed
    • One subunit is bound to cytochrome b558, and this subunit is the one bound to the cytosolic side of the membrane PubMed PubMed
    • Another subunit is the flavoprotein one, required for FAD usage PubMed PubMed
    • The other subunit has an iron-sulphur domain necessary for the catalytic activity PubMed PubMed
  • extensive information on the structure and enzymatic properties of succinate dehydrogenase can be found at Proteopedia

Expression and regulation

  • Regulation:
    • constitutive
    • part of the iron sparing response (FsrA) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP743 (sdhCA, cat), available in Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References