Difference between revisions of "Sda"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
 
* '''Description:''' Sda is a checkpoint protein that is upregulated in response to replication stress [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=11207367%5Buid%5D&log$=activity (Burkholder et al 2001, Cell)]. Sda inhibits the autokinase activity of [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinA KinA] (and likely [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinB KinB] as well). This in its turn results in reduced levels of [http://subtiwiki.uni-goettingen.de/wiki/index.php/Spo0A Spo0A~P]. Thus, replication stressed cells will not iniate sporulation<br/><br/> Sda is rapidly turned over by the [http://subtiwiki.uni-goettingen.de/wiki/index.php/ClpP ClpP/X] system because of some uncharged residues at its C-terminus [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=16796683%5Buid%5D&log$=activity Ruvolo et al 2006, MolMic].
 
* '''Description:''' Sda is a checkpoint protein that is upregulated in response to replication stress [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=11207367%5Buid%5D&log$=activity (Burkholder et al 2001, Cell)]. Sda inhibits the autokinase activity of [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinA KinA] (and likely [http://subtiwiki.uni-goettingen.de/wiki/index.php/KinB KinB] as well). This in its turn results in reduced levels of [http://subtiwiki.uni-goettingen.de/wiki/index.php/Spo0A Spo0A~P]. Thus, replication stressed cells will not iniate sporulation<br/><br/> Sda is rapidly turned over by the [http://subtiwiki.uni-goettingen.de/wiki/index.php/ClpP ClpP/X] system because of some uncharged residues at its C-terminus [http://www.ncbi.nlm.nih.gov/sites/entrez?db=pubmed&cmd=DetailsSearch&term=16796683%5Buid%5D&log$=activity Ruvolo et al 2006, MolMic].
 +
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
Line 32: Line 33:
  
 
__TOC__
 
__TOC__
 +
  
 
<br/><br/>
 
<br/><br/>
 +
  
 
=The gene=
 
=The gene=

Revision as of 14:22, 13 March 2009

  • Description: Sda is a checkpoint protein that is upregulated in response to replication stress (Burkholder et al 2001, Cell). Sda inhibits the autokinase activity of KinA (and likely KinB as well). This in its turn results in reduced levels of Spo0A~P. Thus, replication stressed cells will not iniate sporulation

    Sda is rapidly turned over by the ClpP/X system because of some uncharged residues at its C-terminus Ruvolo et al 2006, MolMic.


Gene name sda
Synonyms
Essential no
Product developmental checkpoint protein
Function mediates a developmental checkpoint coupling initiation of

sporulation (phosphorylation of Spo0A) to the function

of replication initiation proteins

MW, pI 6 kDa, 5.605
Gene length, protein length 156 bp, 52 aa
Immediate neighbours yloV, sdaAA
Gene sequence (+200bp) Protein sequence
Genetic context
Sda context.gif





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed