scpB

168

part of the [wiki|condensin] complex, chromosomal origin condensation and segregation

locus: BSU_23210

Molecular weight: 21.89 kDa

pI: 4.25

Protein length: 197 aa

Gene length: 594 bp

function: segregation of replication origins

product: DNA segregation and condensation protein

essential: no

synonyms: scpB, ypuH, spcB

List of homologs in different organisms, belongs to COG1386 (Galperin et al., 2021)

Gene

Coordinates: 2,425,248  2,425,841

Phenotypes of a mutant

• essential [Pubmed|24440399], non-essential according to [Pubmed|28189581]
• ''[gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]'' mutants are not viable on complex medim that allow rapid growth, but they are viable under conditions of slow growth [Pubmed|24440399,24440393]

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The protein

Catalyzed reaction/ biological activity

• negatively regulates binding of the [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] NTD to the [protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc] neck region [pubmed|28286005]

Protein family

• scpB family (single member, according to UniProt)

Structure

• [PDB|4I98] (complex between [protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA] (aa 1-160)-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] (aa 1-183) [Pubmed|23353789]
• [AF|P35155]

[wiki|Localization]

• nucleoid (Multiple) [Pubmed|16479537]
• the [wiki|condensin] ([protein|0136394A52759CEEDD8DE074ED74033C6FFB8435|smc])2-[protein|8DD968C97FDC0E81293887E2FCB7EE1A9416A333|scpA]-[protein|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB] complex is loaded by [protein|EF4BD49FCD49EE97908973581478951C8FA196C0|parB] to parS centromeric sites adjacent to the replication origin, travels then from the origin to the terminus at rates >50 kb per minute [Pubmed|28154080,24440393,23475963]

Biological materials

Mutant

• MGNA-A105 (ypuH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/105 NBRP B. subtilis, Japan]
• BKE23210 ([gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG
• BKK23210 ([gene|9137D162E6331E2B127472BCF5F49A9AE46266F3|scpB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23210 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACAATAGCTTTCCAATTCA,  downstream forward: _UP4_TAGAAGTTTAATGAATCGCG

References

Reviews

• 22933559,22934648,24118085,25460803,26706151,29182912,27075410,34220773

Original Publications

• 12100548,12065423,12421306,22385855,16479537,19450516,12897137,7934830,11948165,23353789,24440399,24440393,25071173,25557547,25951515,26253537,23475963,26295962,26725510,26904953,28189581,28154080,28286005,28689660,30192981,31201090,31175837,32250245,33472056,33910021,34312537,34346312,35474142