scoC

168

transcriptional repressor of genes expressed in the transition phase

locus: BSU_09990

Molecular weight: 23.57 kDa

pI: 5.19

Protein length: 203 aa

Gene length: 612 bp

function: transition state regulator

product: transcriptional repressor ([wiki|MarR family])

essential: no

synonyms: scoC, hpr, catA

List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)

Gene

Coordinates: 1,073,106 → 1,073,717

additional information

• the gene readily acquires mutations during cultivation with Arabidopsis thaliana roots [pubmed|37768063]

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The protein

[wiki|Domains]

• [wiki|HTH marR-type domain] (aa 13-157) (according to UniProt)

Structure

• [PDB|2FXA]
• [AF|P11065]

Modification

• phosphorylated on Arg-3 [Pubmed|22517742]

Effectors of protein activity

• activity is inhibited in an unknown way by [protein|83F1BE86A7AE1098C1319198A8DDA6B8C5F49236|prkA] (possibly phosphorylation) [Pubmed|25983726]
• DNA binding is affected by [protein|D3F00747006D974A8635D4391E0C261A3276E6AB|dnmA]-mediated DNA methylation [pubmed|32324221]

Biological materials

Mutant

• 1A918 ( ''scoC''::''erm  trpC2 leuC7''), [Pubmed|15126467], available at the [http://bgsc.org/getdetail.php?bgscid=1A918 Bacillus Genetic Stock Center]
• BKE09990 (Δ[gene|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE09990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC,  downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA
• BKK09990 (Δ[gene|F7FD94CE7AB290FBFD3A9B88F204961668F9B42C|scoC]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK09990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTACGTCACCTGCTTCTC,  downstream forward: _UP4_GAAGAGCTCGAACCTGTAAA

Expression vectors

• for expression, purification in E. coli with N-terminal His-tag, pRSETA available in [wiki|Ulf Gerth]'s lab

References

Reviews

• 28886686

Original Publications

• 3131303,16923912,1906467,10383984,16321961,14629015,1907636,15126467,2504584,15104138,19118355,19801406,19898538,20382764,22517742,23660663,11717292,25966844,19251843,26473603,25983726,26728191,31039790,32324221,37768063,38260623