Difference between revisions of "ScoB"

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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[yxjC]]-[[scoA]]-[[scoB]]-[[yxjF]]'' {{PubMed|10746760}}
  
* '''Operon:''' ''[[yxjC]]-[[scoA]]-[[scoB]]-[[yxjF]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/10746760 PubMed]
+
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|12662922}}
 
 
* '''[[Sigma factor]]:''' [[SigE]] {{PubMed|15699190}}
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]]) {{PubMed|12850135,10666464}} 
 +
** expressed early during sporulation in the mother cell ([[SigE]]) {{PubMed|12662922}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression {{PubMed|12850135,10666464}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed>17363272,,16672620,, </pubmed>
+
<pubmed>17363272,10746760,16672620,12662922,12850135,10666464 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:33, 7 February 2010

  • Description: probable succinyl CoA:3-oxoacid CoA-transferase (subunit B)

Gene name scoB
Synonyms yxjE
Essential no
Product probable succinyl CoA:3-oxoacid CoA-transferase (subunit B)
Function lipid metabolism
MW, pI 23 kDa, 4.893
Gene length, protein length 648 bp, 216 aa
Immediate neighbours yxjF, scoA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ScoB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU38980

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Succinyl-CoA + a 3-oxo acid = succinate + a 3-oxoacyl-CoA (according to Swiss-Prot)
  • Protein family: 3-oxoacid CoA-transferase subunit B family (according to Swiss-Prot)
  • Paralogous protein(s): YodR

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed by glucose (CcpA) PubMed
    • expressed early during sporulation in the mother cell (SigE) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Lucy Stols, Min Zhou, William H Eschenfeldt, Cynthia Sanville Millard, James Abdullah, Frank R Collart, Youngchang Kim, Mark I Donnelly
New vectors for co-expression of proteins: structure of Bacillus subtilis ScoAB obtained by high-throughput protocols.
Protein Expr Purif: 2007, 53(2);396-403
[PubMed:17363272] [WorldCat.org] [DOI] (P p)

Juliane Ollinger, Kyung-Bok Song, Haike Antelmann, Michael Hecker, John D Helmann
Role of the Fur regulon in iron transport in Bacillus subtilis.
J Bacteriol: 2006, 188(10);3664-73
[PubMed:16672620] [WorldCat.org] [DOI] (P p)

Hans-Matti Blencke, Georg Homuth, Holger Ludwig, Ulrike Mäder, Michael Hecker, Jörg Stülke
Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways.
Metab Eng: 2003, 5(2);133-49
[PubMed:12850135] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Shane T Jensen, Erin M Conlon, Christiaan van Ooij, Jessica Silvaggi, José Eduardo González-Pastor, Masaya Fujita, Sigal Ben-Yehuda, Patrick Stragier, Jun S Liu, Richard Losick
The sigmaE regulon and the identification of additional sporulation genes in Bacillus subtilis.
J Mol Biol: 2003, 327(5);945-72
[PubMed:12662922] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)