SasA

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  • Description: (p)ppGpp synthetase, small alarmone synthetase

Gene name ywaC
Synonyms ipa-7d
Essential no
Product (p)ppGpp synthetase
Function ppGpp synthesis independent from stringent response
MW, pI 24 kDa, 6.217
Gene length, protein length 630 bp, 210 aa
Immediate neighbours ywaD, menA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YwaC context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

metabolism of signalling nucleotides, cell envelope stress proteins (controlled by SigM, W, X, Y)

This gene is a member of the following regulons

SigM regulon SigW regulon

The gene

Basic information

  • Locus tag: BSU38480

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s): YjbM

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: Transcription is highly upregulated in response to lesions in late-acting teichoic acid synthesis genes tagB, tagD and tagF, and also in response to cell-wall-active compounds like bacitracin or vancomycin PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Michael A D'Elia, Kathryn E Millar, Amit P Bhavsar, Ana M Tomljenovic, Bernd Hutter, Christoph Schaab, Gabriel Moreno-Hagelsieb, Eric D Brown
Probing teichoic acid genetics with bioactive molecules reveals new interactions among diverse processes in bacterial cell wall biogenesis.
Chem Biol: 2009, 16(5);548-56
[PubMed:19477419] [WorldCat.org] [DOI] (I p)

Yousuke Natori, Kazumi Tagami, Kana Murakami, Sawako Yoshida, Osamu Tanigawa, Yoonsuh Moh, Kenta Masuda, Tetsuya Wada, Shota Suzuki, Hideaki Nanamiya, Yuzuru Tozawa, Fujio Kawamura
Transcription activity of individual rrn operons in Bacillus subtilis mutants deficient in (p)ppGpp synthetase genes, relA, yjbM, and ywaC.
J Bacteriol: 2009, 191(14);4555-61
[PubMed:19447912] [WorldCat.org] [DOI] (I p)

Anjana Srivatsan, Yi Han, Jianlan Peng, Ashley K Tehranchi, Richard Gibbs, Jue D Wang, Rui Chen
High-precision, whole-genome sequencing of laboratory strains facilitates genetic studies.
PLoS Genet: 2008, 4(8);e1000139
[PubMed:18670626] [WorldCat.org] [DOI] (I e)

Hideaki Nanamiya, Koji Kasai, Akira Nozawa, Choong-Soo Yun, Takakuni Narisawa, Kana Murakami, Yousuke Natori, Fujio Kawamura, Yuzuru Tozawa
Identification and functional analysis of novel (p)ppGpp synthetase genes in Bacillus subtilis.
Mol Microbiol: 2008, 67(2);291-304
[PubMed:18067544] [WorldCat.org] [DOI] (P p)

Min Cao, Tao Wang, Rick Ye, John D Helmann
Antibiotics that inhibit cell wall biosynthesis induce expression of the Bacillus subtilis sigma(W) and sigma(M) regulons.
Mol Microbiol: 2002, 45(5);1267-76
[PubMed:12207695] [WorldCat.org] [DOI] (P p)

Min Cao, Phil A Kobel, Maud M Morshedi, Ming Fang Winston Wu, Chris Paddon, John D Helmann
Defining the Bacillus subtilis sigma(W) regulon: a comparative analysis of promoter consensus search, run-off transcription/macroarray analysis (ROMA), and transcriptional profiling approaches.
J Mol Biol: 2002, 316(3);443-57
[PubMed:11866510] [WorldCat.org] [DOI] (P p)