Sandbox

• Description: dipeptide ABC transporter (dipeptide-binding protein)
  
  

Gene name	dppE
Synonyms	dciAE
Essential	no
Product	dipeptide ABC transporter (dipeptide-binding protein)
Function	uptake of dipeptides
MW, pI	62 kDa, 5.523
Gene length, protein length	1647 bp, 549 aa
Immediate neighbours	dppD, ykfA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU12960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 5 family (according to Swiss-Prot)

• Paralogous protein(s): OppA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: membrane protein PubMed

Database entries

• Structure:

• Swiss prot entry: P26906

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: dppA-dppB-dppC-dppD-dppE PubMed

• Sigma factor: SigA PubMed

• Regulation: expressed postexponentially (AbrB) PubMed, repressed by CodY PubMed
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • repressed during logrithmic growth (AbrB) PubMed

• Regulatory mechanism: AbrB: transcription repression PubMed, CodY: transcription repression PubMed
  • CodY: transcription repression PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant: GP1110 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

F J Slack, J P Mueller, M A Strauch, C Mathiopoulos, A L Sonenshein
Transcriptional regulation of a Bacillus subtilis dipeptide transport operon.
Mol Microbiol: 1991, 5(8);1915-25
[PubMed:1766371] [WorldCat.org] [DOI] (P p)

1. Hahne et al. (2008) From complementarity to comprehensiveness - targeting the membrane proteome of growing Bacillus subtilis by divergent approaches. Proteomics 8: 4123-4136 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed