Sandbox

• Description: transcriptional repressor of the gluconate operon
  
  

Gene name	gntR
Synonyms
Essential	no
Product	transcriptional repressor of the gluconate operon
Function	regulation of gluconate utilization
MW, pI	28 kDa, 5.26
Gene length, protein length	729 bp, 243 aa
Immediate neighbours	yxaA, gntK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: gluconate acts as molecular inducer

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P10585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: gntR-gntK-gntP-gntZ PubMed1PubMed2

• Sigma factor: SigA PubMed

• Regulation:
  • induced by gluconate (GntR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • GntR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

K Yoshida, Y Miwa, H Ohmori, Y Fujita
Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations.
Mol Gen Genet: 1995, 248(5);583-91
[PubMed:7476858] [WorldCat.org] [DOI] (P p)

A Reizer, J Deutscher, M H Saier, J Reizer
Analysis of the gluconate (gnt) operon of Bacillus subtilis.
Mol Microbiol: 1991, 5(5);1081-9
[PubMed:1659648] [WorldCat.org] [DOI] (P p)

Y Fujita, T Fujita, Y Miwa, J Nihashi, Y Aratani
Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis.
J Biol Chem: 1986, 261(29);13744-53
[PubMed:3020045] [WorldCat.org] (P p)

1. Yoshida K, Miwa Y, Ohmori H, Fujita Y. (1995) Analysis of an insertional operator mutation (gntOi) that affects the expression level of the Bacillus subtilis gnt operon, and characterization of gntOi suppressor mutations. Mol Gen Genet. Sep 20;248(5): 583-91. PubMed
2. Fujita Y, Fujita T, Miwa Y, Nihashi J, Aratani Y. (1986) Organization and transcription of the gluconate operon, gnt, of Bacillus subtilis. J Biol Chem. Oct 15;261(29): 13744-53. PubMed
3. Reizer A., Deutscher J., Saier M.H. Jr., Reizer J. (1991) Analysis of the gluconate (gnt) operon of Bacillus subtilis. Mol. Microbiol. 5: 1081-1089. PubMed