Sandbox

• Description: replication initiation protein
  
  

Gene name	dnaA
Synonyms	dnaH, dnaJ, dnaK
Essential	yes
Product
Function	initiation of chromosome replication
MW, pI	50 kDa, 6.035
Gene length, protein length	1338 bp, 446 aa
Immediate neighbours
Gene sequence (+200bp)	Protein sequence
Genetic context

The gene

Basic information

• Coordinates:

Phenotypes of a mutant

essential

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: DnaA-YabA-DnaN PubMed, DnaA-DnaD PubMed

• Localization: throughout the cytoplasm PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry:

• E.C. number:

Expression and regulation

• Operon: dnaA-dnaN PubMed

• Sigma factor: SigA PubMed

• Regulation: negatively controlled by DnaA PubMed and Spo0A PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Philippe Noirot, Jouy-en-Josas, France homepage

References

1. Imai et al. (2000) Subcellular localization of Dna-initiation proteins of Bacillus subtilis: evidence that chromosome replication begins at either edge of the nucleoids. Mol. Microbiol. 36: 1037-1048. PubMed
2. Ishigo-Oka et al. (2001) DnaD protein of Bacillus subtilis interacts with DnaA, the initiator protein of replication. J. Bacteriol. 183: 2148-2150. PubMed
3. Noirot-Gros et al. (2002) An expanded view of bacterial DNA replication. Proc. Natl. Acad. Sci. USA 99: 8342-8347. PubMed
4. Noirot-Gros et al. (2002) Functional dissection of YabA, a negative regulator of DNA replication initiation in Bacillus Title Proc. Natl. Acad. Sci. USA 103: 2368-2373. PubMed
5. Ogasawara et al. (1985) Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.Nucl. Acids Res. 11: 2267-2279. PubMed
6. Fukuoka et al. (1990) Purification and characterization of an initiation protein for chromosomal replication, DnaA, in Bacillus subtilis. J. Biochem. 107: 732-739. PubMed
7. Molle et al. (2003) The Spo0A regulon of Bacillus subtilis.Mol. Microbiol. 50: 1683-1701. PubMed
8. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed