Difference between revisions of "Sandbox"

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* '''Description:''' deoxyadenosin/deoxycytidine kinase <br/><br/>
+
* '''Description:''' deoxyguanosine kinase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
−
|''dck''
+
|''dgk''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaF ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yaaG ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Product''' || deoxyadenosin/deoxycytidine kinase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || deoxyguanosine kinase
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion
 
|style="background:#ABCDEF;" align="center"|'''Function''' || purine salvage and interconversion
 
|-
 
|-
−
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00140 dck]
+
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00150 dgk]
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/purines_salvage_pathways.html Purine salvage], [http://subtiwiki.uni-goettingen.de/pathways/gene_regulation_nucleotides.html Nucleotides (regulation)]'''
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 25 kDa, 5.536  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 5.783  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 651 bp, 217 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 621 bp, 207 aa  
 
|-
 
|-
−
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[trnSL-Ser1]]'', ''[[dgk]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[dck]]'', ''[[yaaH]]''
 
|-
 
|-
−
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00140 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00140 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00140 DNA_with_flanks]
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00150 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00150 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00150 DNA_with_flanks]
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:dck_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:dgk_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
−
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dck_22496_23149_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:dck_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00140]]
+
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dgk_23146_23769_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:dgk_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00150]]
 
|-
 
|-
 
|}
 
|}
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=== Basic information ===
 
=== Basic information ===
  
−
* '''Locus tag:''' BSU00140
+
* '''Locus tag:''' BSU00150
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dgk-dck.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dgk-dck.html]
  
−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10078]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10079]
  
 
=== Additional information===
 
=== Additional information===
Line 98: Line 98:
 
* '''Structure:'''
 
* '''Structure:'''
  
−
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37529 P37529]
+
* '''UniProt:''' [http://www.uniprot.org/uniprot/P37530 P37530]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00140]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00150]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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* '''Operon:''' ''[[dgk]]-[[dck]]''
 
* '''Operon:''' ''[[dgk]]-[[dck]]''
−
 
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dgk_23146_23769_-1 dgk] {{PubMed|22383849}}
−
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dck_22496_23149_-1 dck] {{PubMed|22383849}}
 
  
 
* '''Sigma factor:'''  
 
* '''Sigma factor:'''  
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=References=
 
=References=
−
<pubmed>6251049, 11078735 </pubmed>
+
<pubmed>11078735 </pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 15:54, 11 November 2013

  • Description: deoxyguanosine kinase

Gene name dgk
Synonyms yaaG
Essential no
Product deoxyguanosine kinase
Function purine salvage and interconversion
Gene expression levels in SubtiExpress: dgk
Metabolic function and regulation of this protein in SubtiPathways:
Purine salvage, Nucleotides (regulation)
MW, pI 23 kDa, 5.783
Gene length, protein length 621 bp, 207 aa
Immediate neighbours dck, yaaH
Sequences Protein DNA DNA_with_flanks
Genetic context
Dgk context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Dgk expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU00150

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

R B Andersen, J Neuhard
Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donor.
J Biol Chem: 2001, 276(8);5518-24
[PubMed:11078735] [WorldCat.org] [DOI] (P p)

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