Difference between revisions of "Sandbox"

From SubtiWiki
Jump to: navigation, search
Line 1: Line 1:
* '''Description:''' modifier protein of major autolysin [[LytC]] <br/><br/>
+
* '''Description:''' pyrrolidone-carboxylate peptidase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''lytB''
+
|''pcp''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cwbA ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || modifier protein of major autolysin LytC
+
|style="background:#ABCDEF;" align="center"| '''Product''' || pyrrolidone-carboxylate peptidase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || autolysis
+
|style="background:#ABCDEF;" align="center"|'''Function''' || removal of the N-terminal pyroglutamyl group from peptides
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 9.835  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23 kDa, 6.418  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 2115 bp, 705 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 645 bp, 215 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[lytC]]'', ''[[lytA]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[tatCD]]'', ''[[ycbU]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15580&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12059&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:lytB_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:pcp_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 29: Line 29:
 
__TOC__
 
__TOC__
  
<br/><br/><br/>
+
<br/><br/><br/><br/>
  
 
=The gene=
 
=The gene=
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU35630
+
* '''Locus tag:''' BSU02650
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/lytABC.html]
+
* '''DBTBS entry:''' no entry
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10406]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10873]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:'''  
+
* '''Catalyzed reaction/ biological activity:''' Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to Swiss-Prot)
  
* '''Protein family:'''
+
* '''Protein family:''' peptidase C15 family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 72: Line 72:
 
* '''Interactions:'''
 
* '''Interactions:'''
  
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:''' cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
Line 78: Line 78:
 
* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q02113 Q02113]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P28618 P28618]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU35630]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU02650]
  
* '''E.C. number:'''
+
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.4.11.8 3.4.11.8]
  
 
=== Additional information===
 
=== Additional information===
Line 88: Line 88:
 
=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[lytA]]-[[lytB]]-[[lytC]]''
+
* '''Operon:'''  
  
* '''[[Sigma factor]]:''' [[SigA]], [[SigD]]
+
* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
  
* '''Regulatory mechanism:''' repressed by [[YvrHb]]  [http://www.ncbi.nlm.nih.gov/sites/entrez/16306698 PubMed]
+
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
<pubmed>1355454,, </pubmed>
+
<pubmed>1362573,1353026,, </pubmed>
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 08:57, 13 June 2009

  • Description: pyrrolidone-carboxylate peptidase

Gene name pcp
Synonyms
Essential no
Product pyrrolidone-carboxylate peptidase
Function removal of the N-terminal pyroglutamyl group from peptides
MW, pI 23 kDa, 6.418
Gene length, protein length 645 bp, 215 aa
Immediate neighbours tatCD, ycbU
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
Pcp context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Locus tag: BSU02650

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Release of an N-terminal pyroglutamyl group from a polypeptide, the second amino acid generally not being Pro (according to Swiss-Prot)
  • Protein family: peptidase C15 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cytoplasm (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

T Gonzalès, A Awadé, C Besson, J Robert-Baudouy
Purification and characterization of recombinant pyrrolidone carboxyl peptidase of Bacillus subtilis.
J Chromatogr: 1992, 584(1);101-7
[PubMed:1362573] [DOI] (P p)

A Awadé, P Cleuziat, T Gonzalès, J Robert-Baudouy
Characterization of the pcp gene encoding the pyrrolidone carboxyl peptidase of Bacillus subtilis.
FEBS Lett: 1992, 305(1);67-73
[PubMed:1353026] [WorldCat.org] [DOI] (P p)

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed