Difference between revisions of "Sandbox"

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* '''Description:''' [[penicillin-binding protein]] 5*, D-alanyl-D-alanine carboxypeptidase <br/><br/>
+
#REDIRECT [[PdxS]]
 
 
{| align="right" border="1" cellpadding="2"
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|''dacA''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Product''' || [[penicillin-binding protein]] 5*, <br/>D-alanyl-D-alanine carboxypeptidase
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || carboxypeptidase
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00100 dacA]
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 48 kDa, 5.668 
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1329 bp, 443 aa
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[guaB]]'', ''[[pdxS]]''
 
|-
 
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU00100 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU00100 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU00100 DNA_with_flanks]
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:dacA_context.gif]]
 
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dacA_17534_18865_1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:dacA_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU00100]]
 
|-
 
|}
 
 
 
__TOC__
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/><br/><br/>
 
<br/><br/>
 
 
 
= [[Categories]] containing this gene/protein =
 
{{SubtiWiki category|[[cell wall synthesis]]}},
 
{{SubtiWiki category|[[membrane proteins]]}}
 
 
 
= This gene is a member of the following [[regulons]] =
 
 
 
 
 
=The gene=
 
 
 
=== Basic information ===
 
 
 
* '''Locus tag:''' BSU00100
 
 
 
===Phenotypes of a mutant ===
 
 
 
=== Database entries ===
 
 
 
* '''DBTBS entry:''' no entry
 
 
 
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10074]
 
 
 
=== Additional information===
 
 
 
 
 
 
 
 
 
=The protein=
 
 
 
=== Basic information/ Evolution ===
 
 
 
* '''Catalyzed reaction/ biological activity:''' Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala (according to Swiss-Prot)
 
 
 
* '''Protein family:''' peptidase S11 family (according to Swiss-Prot)
 
 
 
* '''Paralogous protein(s):'''
 
 
 
=== Extended information on the protein ===
 
 
 
* '''Kinetic information:'''
 
 
 
* '''Domains:'''
 
 
 
* '''Modification:'''
 
 
 
* '''Cofactor(s):'''
 
 
 
* '''Effectors of protein activity:'''
 
 
 
* '''[[SubtInteract|Interactions]]:'''
 
 
 
* '''[[Localization]]:'''
 
** secreted (according to Swiss-Prot),  membrane associated [http://www.ncbi.nlm.nih.gov/pubmed/18763711 PubMed]
 
 
 
=== Database entries ===
 
 
 
* '''Structure:'''
 
 
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P08750 P08750]
 
 
 
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU00100]
 
 
 
* '''E.C. number:'''
 
 
 
=== Additional information===
 
 
 
=Expression and regulation=
 
 
 
* '''Operon:'''
 
 
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=dacA_17534_18865_1 dacA] {{PubMed|22383849}}
 
 
 
* '''[[Sigma factor]]:'''
 
 
 
* '''Regulation:'''
 
 
 
* '''Regulatory mechanism:'''
 
 
 
* '''Additional information:'''
 
 
 
=Biological materials =
 
 
 
* '''Mutant:'''
 
** 1A742 ( ''dacA''::''cat''), {{PubMed|3087956}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A742&Search=1A742 BGSC]
 
** 1A743 ( ''dacA''::''cat''), {{PubMed|3087956}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A743&Search=1A743 BGSC]
 
 
 
* '''Expression vector:'''
 
       
 
* '''lacZ fusion:'''
 
 
 
* '''GFP fusion:'''
 
 
 
* '''two-hybrid system:'''
 
 
 
* '''Antibody:'''
 
 
 
=Labs working on this gene/protein=
 
 
 
=Your additional remarks=
 
 
 
=References=
 
 
 
<pubmed>,9864321,18763711, 23531131 </pubmed>
 
 
 
[[Category:Protein-coding genes]]
 

Revision as of 14:54, 11 November 2013

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