Difference between revisions of "Sandbox"

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* '''Description:''' flagella-associated protein <br/><br/>
+
* '''Description:''' flagellar motor switch protein <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''flhF''
+
|''fliY''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''cheD ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || flagella-associated protein
+
|style="background:#ABCDEF;" align="center"| '''Product''' || flagellar motor switch protein
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis  
 
|style="background:#ABCDEF;" align="center"|'''Function''' || movement and chemotaxis  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 8.719  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 40 kDa, 4.117  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1098 bp, 366 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1134 bp, 378 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[flhA]]'', ''[[swrC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[fliM]]'', ''[[cheY]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13513&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB13505&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:flhF_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:fliY_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
Line 35: Line 35:
 
=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU16400
+
* '''Locus tag:''' BSU16320
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 43: Line 43:
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/fla-che.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10544]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10252]
  
 
=== Additional information===
 
=== Additional information===
Line 54: Line 54:
 
* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' GTP-binding SRP family (according to Swiss-Prot)
+
* '''Protein family:''' fliN/mopA/spaO family (according to Swiss-Prot)
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
Line 76: Line 76:
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2PX3 2PX3] (complexed with GTP-Mg2+)
+
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/Q01960 Q01960]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P24073 P24073]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16400]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU16320]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 120: Line 120:
 
=References=
 
=References=
  
<pubmed>1447978,17699634,,15317803,14651647, </pubmed>
+
<pubmed>14749334,1447979,,14651647, </pubmed>
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 09:01, 13 June 2009

  • Description: flagellar motor switch protein

Gene name fliY
Synonyms cheD
Essential no
Product flagellar motor switch protein
Function movement and chemotaxis
MW, pI 40 kDa, 4.117
Gene length, protein length 1134 bp, 378 aa
Immediate neighbours fliM, cheY
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FliY context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU16320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: fliN/mopA/spaO family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed