Difference between revisions of "Sandbox"

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* '''Description:''' synthesis of spore killing factor <br/><br/>
+
* '''Description:''' may be involved in spore killing <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''skfB''
+
|''skfC''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybcP, ybcQ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ybcS''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
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|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || unknown  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || synthesis of spore killing factor
+
|style="background:#ABCDEF;" align="center"|'''Function''' || may be involved in spore killing
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 45 kDa, 4.934  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 21 kDa, 8.433  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1230 bp, 410 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 555 bp, 185 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfA]]'', ''[[skfC]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[skfB]]'', ''[[skfD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB11985&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAX52541&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:ybcP_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:ybcS_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU01920
+
* '''Locus tag:''' BSU01930
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/skf.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/skf.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12714]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG12716]
  
 
=== Additional information===
 
=== Additional information===
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* '''Catalyzed reaction/ biological activity:'''  
 
* '''Catalyzed reaction/ biological activity:'''  
  
* '''Protein family:''' moaA/nifB/pqqE family (according to Swiss-Prot)
+
* '''Protein family:'''
  
 
* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
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* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31423 O31423]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O31425 O31425]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU01920]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU01930]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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** [[AbrB]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
 
** [[AbrB]]:  transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/15687200,17720793 PubMed]
  
* '''Additional information:''' Northern blotting during during phosphate limitation showed an intense 0.25 kb ''skfA''-specific transcript, and a weaker 6.5 kb ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfD]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' transcript. The ''[[skfA]]'' transcript is stabilized by a stem-loop structure in the ''[[skfA]]-[[skfB]]'' intergenic region. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
+
* '''Additional information:''' Northern blotting during during phosphate limitation showed an intense 0.25 kb ''skfA''-specific transcript, and a weaker 6.5 kb ''[[skfA]]-[[skfB]]-[[skfC]]-[[skfD]]-[[skfE]]-[[skfF]]-[[skfG]]-[[skfH]]'' transcript. [http://www.ncbi.nlm.nih.gov/pubmed/16816204 PubMed]
  
 
=Biological materials =
 
=Biological materials =

Revision as of 14:48, 11 June 2009

  • Description: may be involved in spore killing

Gene name skfC
Synonyms ybcS
Essential no
Product unknown
Function may be involved in spore killing
MW, pI 21 kDa, 8.433
Gene length, protein length 555 bp, 185 aa
Immediate neighbours skfB, skfD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YbcS context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU01930

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information: Northern blotting during during phosphate limitation showed an intense 0.25 kb skfA-specific transcript, and a weaker 6.5 kb skfA-skfB-skfC-skfD-skfE-skfF-skfG-skfH transcript. PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed