Difference between revisions of "Sandbox"

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* '''Description:''' phosphodiesterase/alkaline phosphatase <br/><br/>
+
* '''Description:''' xanthine dehydrogenase <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''phoD''
+
|''pucE''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ycbS ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yurB ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || phosphodiesterase/alkaline phosphatase
+
|style="background:#ABCDEF;" align="center"| '''Product''' || xanthine dehydrogenase
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || aquisition of phosphate upon phosphoate starvation 
+
|style="background:#ABCDEF;" align="center"|'''Function''' || purine utilization
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 62 kDa, 8.394  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 18 kDa, 6.127  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1668 bp, 556 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 519 bp, 173 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ycbR]]'', ''[[tatAD]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[pucM]]'', ''[[pucD]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB12056&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB15237&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:phoD_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:pucE_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU02620
+
* '''Locus tag:''' BSU32470
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/phoD.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/pucABCDE.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11174]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13988]
  
 
=== Additional information===
 
=== Additional information===
Line 52: Line 52:
 
=== Basic information/ Evolution ===
 
=== Basic information/ Evolution ===
  
* '''Catalyzed reaction/ biological activity:''' A phosphate monoester + H<sub>2</sub>O = an alcohol + phosphate (according to Swiss-Prot)  
+
* '''Catalyzed reaction/ biological activity:''' Xanthine + NAD<sup>+</sup> + H<sub>2</sub>O = urate + NADH (according to Swiss-Prot)  
  
 
* '''Protein family:'''
 
* '''Protein family:'''
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[TatAD]]-[[PhoD]]
+
* '''Interactions:'''
  
* '''Localization:''' extracellular (signal peptide) [http://www.ncbi.nlm.nih.gov/pubmed/18957862 PubMed], secreted by the [[TatAD]]-[[TatCD]] complex  [http://www.ncbi.nlm.nih.gov/sites/entrez/19395490 PubMed]
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
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* '''Structure:'''
 
* '''Structure:'''
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P42251 P42251]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O32143 O32143]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU02620]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU32470]
  
* '''E.C. number:''' [http://www.expasy.org/enzyme/3.1.3.1 3.1.3.1]
+
* '''E.C. number:'''
  
 
=== Additional information===
 
=== Additional information===
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 
[[Oscar Kuipers]], University of Groningen, The Netherlands
 
[http://molgen.biol.rug.nl/molgen/index.php Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
 
+
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
<pubmed> 18957862, 19383693, 19395490 </pubmed>
 

Revision as of 13:08, 11 June 2009

  • Description: xanthine dehydrogenase

Gene name pucE
Synonyms yurB
Essential no
Product xanthine dehydrogenase
Function purine utilization
MW, pI 18 kDa, 6.127
Gene length, protein length 519 bp, 173 aa
Immediate neighbours pucM, pucD
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
PucE context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU32470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed