Difference between revisions of "Sandbox"
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− | * '''Description:''' RNA polymerase [[sigma factor]] | + | * '''Description:''' RNA polymerase forespore-specific (early) [[sigma factor]] SigF <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |'' | + | |''sigF'' |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''spoIIAC '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase [[sigma factor]] | + | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase forespore-specific <br/>(early) [[sigma factor]] SigF |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || transcription of sporulation genes (late mother cell) |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5. | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.078 |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 765 bp, 255 aa |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[spoVAA]]'', ''[[spoIIAB]]'' |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB14277]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
− | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:sigF_context.gif]] |
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
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__TOC__ | __TOC__ | ||
− | <br/><br/> | + | <br/><br/><br/> |
=The gene= | =The gene= | ||
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=== Basic information === | === Basic information === | ||
− | * '''Locus tag:''' | + | * '''Locus tag:''' BSU23450 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
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=== Database entries === | === Database entries === | ||
− | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/dacF-spoIIAAB-sigF.html] |
− | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10298] |
=== Additional information=== | === Additional information=== | ||
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* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' | + | * '''Protein family:''' sigma-70 factor family (according to Swiss-Prot) |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
− | === Genes | + | === Genes controlled by SigF === |
− | ''[[ | + | ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'', ''[[sigG]]'','' [[pbpF]]'', ''[[yphA]]-[[seaA]]'' |
=== Extended information on the protein === | === Extended information on the protein === | ||
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* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' [[ | + | * '''Interactions:''' [[SigF]]-[[SpoIIAB]] |
− | * '''Localization:''' | + | * '''Localization:''' cell membrane (according to Swiss-Prot) |
=== Database entries === | === Database entries === | ||
− | * '''Structure:''' | + | * '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=1L0O 1L0O] (complex with SpoIIAB, Geobacillus stearothermophilus) |
− | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/ | + | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P07860 P07860] |
− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU23450] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' | + | * '''Operon:''' ''[[dacF]]-[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' |
− | + | ''[[spoIIAA]]-[[spoIIAB]]-[[sigF]]'' | |
− | |||
− | |||
− | |||
− | |||
− | |||
− | * ''' | + | * '''[[Sigma factor]]:''' ''[[dacF]]'': [[SigF]], [[SigG]] |
− | + | ''[[spoIIAA]]'': [[SigH]] | |
+ | |||
+ | * '''Regulation:''' repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] , ''[[dacF]]'': expressed during sporulation, ''[[spoIIAA]]'': expressed early during sporulation | ||
+ | |||
+ | * '''Regulatory mechanism:''' ''[[spoIIAA]]'': activated by [[Spo0A]] and repressed by [[SinR]] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
− | * [[ | + | * [[Charles Moran]], Emory University, NC, USA [http://www.microbiology.emory.edu/moran_c.html homepage] |
− | |||
=Your additional remarks= | =Your additional remarks= | ||
Line 128: | Line 126: | ||
=References= | =References= | ||
− | <pubmed> | + | <pubmed>12107147, </pubmed> |
− | # | + | # Mäder et al. (2002) Transcriptome and Proteome Analysis of ''Bacillus subtilis'' Gene Expression Modulated by Amino Acid Availability. ''J. Bacteriol'' '''184:''' 1844288-4295 [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed] |
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− | |||
− | |||
− | |||
− | |||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
Revision as of 13:36, 8 June 2009
- Description: RNA polymerase forespore-specific (early) sigma factor SigF
Gene name | sigF |
Synonyms | spoIIAC |
Essential | no |
Product | RNA polymerase forespore-specific (early) sigma factor SigF |
Function | transcription of sporulation genes (late mother cell) |
MW, pI | 29 kDa, 5.078 |
Gene length, protein length | 765 bp, 255 aa |
Immediate neighbours | spoVAA, spoIIAB |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU23450
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: sigma-70 factor family (according to Swiss-Prot)
- Paralogous protein(s):
Genes controlled by SigF
dacF-spoIIAA-spoIIAB-sigF, sigG, pbpF, yphA-seaA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure: 1L0O (complex with SpoIIAB, Geobacillus stearothermophilus)
- Swiss prot entry: P07860
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Sigma factor: dacF: SigF, SigG
- Regulation: repressed by casamino acids PubMed , dacF: expressed during sporulation, spoIIAA: expressed early during sporulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Charles Moran, Emory University, NC, USA homepage
Your additional remarks
References
Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147]
[WorldCat.org]
[DOI]
(P p)