Difference between revisions of "Sandbox"

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* '''Description:''' general stress protein, transcriptional activator of the bmrU-bmrR operon <br/><br/>
+
* '''Description:''' general stress protein, controls processing of pro-SigK by SpoIVFB <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''bmrR''
+
|''bofC''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional activator
+
|style="background:#ABCDEF;" align="center"| '''Product''' || forespore regulator of the sigma-K checkpoint
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of multidrug resistance
+
|style="background:#ABCDEF;" align="center"|'''Function''' || control of processing of pro-SigK by SpoIVFB
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 32 kDa, 5.187  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 19 kDa, 5.753  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 834 bp, 278 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 510 bp, 170 aa  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[bmr]]'', ''[[bkdB]]''
+
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ruvA]]'', ''[[csbX]]''
 
|-
 
|-
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14333&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14735&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:bmrR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:bofC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
|-
 
|-
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=== Basic information ===
 
=== Basic information ===
  
* '''Locus tag:''' BSU24020
+
* '''Locus tag:''' BSU27750
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/bmrU-bmr-bmrR.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/csbX-bofC.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10304]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11917]
  
 
=== Additional information===
 
=== Additional information===
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* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:'''
+
* '''Interactions:''' [[BofC]]-[[SpoIVB]],  [[BofC]]-[[SpoIVB]] [http://www.ncbi.nlm.nih.gov/sites/entrez/10931291 PubMed]
  
* '''Localization:''' cell membrane (according to Swiss-Prot)
+
* '''Localization:'''
  
 
=== Database entries ===
 
=== Database entries ===
  
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2BOW 2BOW] (complex with effector),  [http://www.rcsb.org/pdb/explore.do?structureId=1R8E 1R8E] (complex with DNA),  [http://www.rcsb.org/pdb/explore.do?structureId=1BOW 1BOW]
+
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2BW2 2BW2]
  
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P39075 P39075]
+
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O05391 O05391]
  
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU24020]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27750]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
Line 113: Line 113:
  
 
=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 
[[Richard Brennan]], Houston, Texas, USA [http://www.mdanderson.org/departments/biochem/display.cfm?id=556ef368-6c81-4043-b74f350d41dd06cb&method=displayfull&pn=a8427ebd-d0ff-11d4-80fd00508b603a14 Homepage]
 
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 13:10, 8 June 2009

  • Description: general stress protein, controls processing of pro-SigK by SpoIVFB

Gene name bofC
Synonyms
Essential no
Product forespore regulator of the sigma-K checkpoint
Function control of processing of pro-SigK by SpoIVFB
MW, pI 19 kDa, 5.753
Gene length, protein length 510 bp, 170 aa
Immediate neighbours ruvA, csbX
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
BofC context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU27750

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Localization:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

A Petersohn, M Brigulla, S Haas, J D Hoheisel, U Völker, M Hecker
Global analysis of the general stress response of Bacillus subtilis.
J Bacteriol: 2001, 183(19);5617-31
[PubMed:11544224] [WorldCat.org] [DOI] (P p)

  1. Petersohn et al. (2001) Global Analysis of the General Stress Response of Bacillus subtilis. J Bacteriol. 183: 5617-5631 PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed