Difference between revisions of "Sandbox"

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Line 4: Line 4:
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
|''licR''
+
|''rsfA''
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''celR ''
+
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ywfN, ipa-92r ''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
+
|style="background:#ABCDEF;" align="center"| '''Product''' || probable transcriptional regulatory protein
 
|-
 
|-
|style="background:#ABCDEF;" align="center"|'''Function''' || positive regulation of the lichenan operon (licBCAH)
+
|style="background:#ABCDEF;" align="center"|'''Function''' || involved in the prespore-specific regulation of sigma-F-dependent genes
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 73 kDa, 6.115  
+
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 5.13  
 
|-
 
|-
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1923 bp, 641 aa  
+
|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 774 bp, 258 aa  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
 
|-
 
|-
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/licR_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/rsfA_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/licR_protein.txt Protein sequence]'''
+
|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/rsfA_protein.txt Protein sequence]'''
 
|-
 
|-
|colspan="2" | '''Genetic context''' <br/> [[Image:licR_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:rsfA_context.gif]]
 
|-
 
|-
 
|}
 
|}
Line 41: Line 41:
 
=== Database entries ===
 
=== Database entries ===
  
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/licR.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rsfA.html]
  
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG11346]
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10638]
  
 
=== Additional information===
 
=== Additional information===
Line 90: Line 90:
 
* '''Operon:'''  
 
* '''Operon:'''  
  
* '''Sigma factor:''' [[sigB]]
+
* '''Sigma factor:''' [[sigB]]  
  
* '''Regulation:'''
+
* '''Regulation:'''  
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
Line 118: Line 118:
 
=References=
 
=References=
  
# Tobisch, S., P. Glaser, S. Krüger, and M. Hecker. 1997. Identification and characterization of a new ß-glucoside utilization system in ''Bacillus subtilis''. J. Bacteriol. 179:496-506. [http://www.ncbi.nlm.nih.gov/sites/entrez/8990303 PubMed]
 
# Tobisch, S., Stülke, J., & Hecker, M. (1999) Regulation of the ''lic'' operon of ''Bacillus subtilis'' and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis. J. Bacteriol. 181: 4995-5003. [http://www.ncbi.nlm.nih.gov/sites/entrez/10438772 PubMed]
 
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 09:56, 19 February 2009

  • Description: write here

Gene name rsfA
Synonyms ywfN, ipa-92r
Essential no
Product probable transcriptional regulatory protein
Function involved in the prespore-specific regulation of sigma-F-dependent genes
MW, pI 29 kDa, 5.13
Gene length, protein length 774 bp, 258 aa
Immediate neighbours
Gene sequence (+200bp) Protein sequence
Genetic context
RsfA context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure:
  • Swiss prot entry:
  • KEGG entry:
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor: sigB
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed