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| − | * '''Description:''' B subunit of DNA gyrase<br/><br/>
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''gyrB''
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''novA ''
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || yes [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || DNA gyrase (subunit B) (EC 5.99.1.3)
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| − | |-
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || initation of replication cycle and DNA elongation
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 71 kDa, 5.378
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1914 bp, 638 aa
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gyrB_nucleotide.txt Gene sequence (+200bp) ]'''
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/gyrB_protein.txt Protein sequence]'''
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| − | |-
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:gyrB_context.gif]]
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| − | |-
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| − | |}
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| − | __TOC__
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| − | <br/><br/>
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| − |
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Coordinates:'''
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| − |
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| − | ===Phenotypes of a mutant ===
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| − | essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
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| − |
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/yaaA-recF-yaaB-gyrB.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10070]
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| − |
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| − | === Additional information===
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| − |
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| − | =The protein=
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| − |
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:'''
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| − | * '''Protein family:'''
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| − |
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| − | * '''Paralogous protein(s):''' [[GrlB]]
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| − |
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | * '''Interactions:''' [[GyrA]]-GyrB
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| − | * '''Localization:''' nucleoid-associated in actively growing cells [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
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| − |
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| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''Swiss prot entry:'''
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| − | * '''KEGG entry:'''
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| − | * '''E.C. number:'''
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| − |
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[yaaA]]-[[recF]]-[[yaaB]]-[[gyrB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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| − | * '''Sigma factor:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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| − | * '''Regulation:'''
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| − | * '''Regulatory mechanism:'''
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| − | * '''Additional information:''' GyrB is subject to Clp-dependent proteolysis upon glucose starvation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| − |
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| − | =Biological materials =
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| − | * '''Mutant:'''
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| − | * '''Expression vector:'''
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| − |
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''two-hybrid system:'''
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| − | * '''Antibody:'''
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| − | =Labs working on this gene/protein=
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| − | =Your additional remarks=
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| − | =References=
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| − | # Huang et al. (1998) Bipolar localization of Bacillus subtilis topoisomerase IV, an enzyme required for chromosome segregation. ''Proc. Natl. Acad. Sci. USA'' '''95:''' 4652-4657. [http://www.ncbi.nlm.nih.gov/sites/entrez/9539793 PubMed]
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| − | # Gerth et al. (2008) Clp-dependent proteolysis down-regulates central metabolic pathways in glucose-starved Bacillus subtilis. J Bacteriol 190:321-331 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17981983 PubMed]
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| − | # Ogasawara et al. (1985) Structure and function of the region of the replication origin of the ''Bacillus subtilis'' chromosome. IV. Transcription of the oriC region and expression of DNA gyrase genes and other open reading frames.''Nucl. Acids Res.'' '''11:''' 2267-2279. [http://www.ncbi.nlm.nih.gov/sites/entrez/2987848 PubMed]
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