Difference between revisions of "Sandbox"

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* '''Description:''' methionyl-tRNA synthetase <br/><br/>
+
* '''Description:''' tRNA-Ala<br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
 
|style="background:#ABCDEF;" align="center"|'''Gene name'''
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|''metS''
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|''trnA-Ala''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
 
|style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' ''
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|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|style="background:#ABCDEF;" align="center"| '''Essential''' || no  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''Product''' || methionyl-tRNA synthetase (EC 6.1.1.10)
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|style="background:#ABCDEF;" align="center"| '''Product''' || transfer RNA-Ala
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Function''' ||  
 
|style="background:#ABCDEF;" align="center"|'''Function''' ||  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 76 kDa, 4.958 
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|style="background:#ABCDEF;" align="center"| '''Gene length''' || 76 bp
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|-
 
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|style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 1992 bp, 664 aa
 
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' ||  
 
|-
 
|-
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/metS_nucleotide.txt    Gene sequence      (+200bp)  ]'''
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/trnA-Ala_nucleotide.txt    Gene sequence      (+200bp)  ]'''  
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|style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/metS_protein.txt Protein sequence]'''
 
 
|-
 
|-
−
|colspan="2" | '''Genetic context''' <br/> [[Image:metS_context.gif]]
+
|colspan="2" | '''Genetic context''' <br/> [[Image:trnA-Ala_context.gif]]
 
|-
 
|-
 
|}
 
|}
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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
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−
essential [http://www.ncbi.nlm.nih.gov/pubmed/12682299 PubMed]
 
  
 
=== Database entries ===
 
=== Database entries ===
  
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* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/metS.html]
+
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rrnA-16S-trnA-Ile-trnA-Ala-rrnA-23S-rrnA-5S.html]
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−
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG10101]
 
  
−
=== Additional information===
+
* '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG00013]
−
 
 
−
 
 
−
=The protein=
 
−
 
 
−
=== Basic information/ Evolution ===
 
−
 
 
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* '''Catalyzed reaction/ biological activity:'''  
 
−
 
 
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* '''Protein family:'''
 
−
 
 
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* '''Paralogous protein(s):'''
 
−
 
 
−
=== Extended information on the protein ===
 
−
 
 
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* '''Kinetic information:'''
 
−
 
 
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* '''Domains:'''
 
−
 
 
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* '''Modification:'''
 
−
 
 
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* '''Cofactor(s):'''
 
−
 
 
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* '''Effectors of protein activity:'''
 
−
 
 
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* '''Interactions:'''
 
−
 
 
−
* '''Localization:'''
 
−
 
 
−
=== Database entries ===
 
  
 
* '''Structure:'''
 
* '''Structure:'''
  
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* '''Swiss prot entry:'''
+
=== Additional information===
−
 
 
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* '''KEGG entry:'''
 
−
 
 
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* '''E.C. number:'''
 
  
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=== Additional information===
 
  
 
=Expression and regulation=
 
=Expression and regulation=
  
−
* '''Operon:''' ''metS''
+
* '''Operon:''' ''[[rrnA-16S]]-[[trnA-Ile]]-[[trnA-Ala]]-[[rrnA-23S]]-[[rrnA-5S]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/6312418 PubMed]
  
−
* '''Sigma factor:'''  
+
* '''Sigma factor:''' SigA [http://www.ncbi.nlm.nih.gov/sites/entrez/6312418 PubMed]
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
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* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
−
* '''Additional information:'''
+
* '''Additional information:'''  
  
 
=Biological materials =
 
=Biological materials =
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* '''lacZ fusion:'''
 
* '''lacZ fusion:'''
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−
* '''GFP fusion:'''
 
  
 
* '''two-hybrid system:'''  
 
* '''two-hybrid system:'''  
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* '''Antibody:'''
 
* '''Antibody:'''
  
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=Labs working on this gene/protein=
+
=Labs working on this gene/RNA=
  
 
=Your additional remarks=
 
=Your additional remarks=
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=References=
 
=References=
  
 +
# Ogasawara et al. (1983) Structure and organization of rRNA operons in the region of the replication origin of the ''Bacillus subtilis'' chromosome.''Nucl. Acids Res.'' '''11:''' 6301-6318. [http://www.ncbi.nlm.nih.gov/sites/entrez/6312418 PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]

Revision as of 15:26, 11 February 2009

  • Description: tRNA-Ala

Gene name trnA-Ala
Synonyms
Essential no
Product transfer RNA-Ala
Function
Gene length 76 bp
Immediate neighbours
Gene sequence (+200bp)
Genetic context
TrnA-Ala context.gif



The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]
  • Structure:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/RNA

Your additional remarks

References

  1. Ogasawara et al. (1983) Structure and organization of rRNA operons in the region of the replication origin of the Bacillus subtilis chromosome.Nucl. Acids Res. 11: 6301-6318. PubMed
  2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
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