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| − | * '''Description:''' transcriptional repressor of gluconeogenetic genes and of [[sr1]]. repression in the presence of glucose <br/><br/>
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| − | {| align="right" border="1" cellpadding="2"
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| − | |style="background:#ABCDEF;" align="center"|'''Gene name'''
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| − | |''ccpN''
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| − | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''yqzB ''
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| − | |style="background:#ABCDEF;" align="center"| '''Essential''' || no
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| − | |style="background:#ABCDEF;" align="center"| '''Product''' || transcriptional regulator
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| − | |style="background:#ABCDEF;" align="center"|'''Function''' || repressor of genes involved in gluconeogenesis (''[[gapB]]'','' [[pckA]]'') and of ''[[sr1]]''
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| − | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 23.4 kDa, 7.22
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| − | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 636 bp, 212 amino acids
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| − | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[glyS]]'', ''[[yqfL]]''
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ccpN_nucleotide.txt Gene sequence (+200bp) ]'''
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| − | |style="background:#FAF8CC;" align="center"|'''[http://subtiwiki.uni-goettingen.de/ccpN_protein.txt Protein sequence]'''
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| − | |colspan="2" | '''Genetic context''' <br/> [[Image:yqzB_context.gif]]
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| − | |}
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| − | __TOC__
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| − | <br/><br/>
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| − | =The gene=
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| − | === Basic information ===
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| − | * '''Coordinates:''' 2604195 - 2604830
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| − | ===Phenotypes of a mutant ===
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| − | Impaired growth on glucose due to re-routing of carbon from glycolysis to the pentose phosphate pathway [http://www.ncbi.nlm.nih.gov/sites/entrez/18586936 PubMed]
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| − |
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| − | === Database entries ===
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| − | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ccpN-yqfL.html]
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| − | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13768]
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| − | === Additional information===
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| − | === Basic information/ Evolution ===
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| − | * '''Catalyzed reaction/ biological activity:''' transcription repression of the ''[[gapB]]'', ''[[pckA]]'', and ''[[sr1]]'' genes in the presence of glucose [http://www.ncbi.nlm.nih.gov/sites/entrez/2548995 PubMed]
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| − | * '''Protein family:'''
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| − | * '''Paralogous protein(s):'''
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| − |
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| − | === Extended information on the protein ===
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| − | * '''Kinetic information:'''
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| − | * '''Domains:'''
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| − | * '''Modification:'''
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| − | * '''Cofactor(s):'''
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| − | * '''Effectors of protein activity:'''
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| − | ** [[YqfL]] (negative effector of CcpN activity) [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| − | ** ATP enhances CcpN-dependent repression, ADP counteracts the repressing activity of CcpN [http://www.ncbi.nlm.nih.gov/sites/entrez/18511073 PubMed]
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| − | * '''Interactions:'''
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| − | * '''Localization:'''
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| − |
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| − | === Database entries ===
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| − | * '''Structure:'''
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| − | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/O34994]
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| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU25250]
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| − |
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| − | === Additional information===
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| − | =Expression and regulation=
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| − | * '''Operon:''' ''[[ccpN]]''-''[[yqfL]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| − | * '''Sigma factor:'''
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| − | * '''Regulation:''' constitutively expressed [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| − | * '''Additional information:'''
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| − | =Biological materials =
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| − | * '''Mutant:''' DB104 ''ccpN''::[[cat]], available in [[Sabine Brantl]] lab
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| − | * '''Expression vector:'''
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| − | * '''lacZ fusion:'''
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| − | * '''GFP fusion:'''
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| − | * '''Antibody:''' available in [[Sabine Brantl]] lab
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| − | =Labs working on this gene/protein=
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| − | [[Stephane Aymerich |Stephane Aymerich]], Microbiology and Molecular Genetics, INRA Paris-Grignon, France
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| − | [[Sabine Brantl |Sabine Brantl]], Bacterial Genetics, Friedrich-Schiller-University of Jena, Germany [http://www.uni-jena.de/AG_Bakteriengenetik_(_Doz__Dr__Sabine_Brantl_).html homepage]
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| − | =Your additional remarks=
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| − | =References=
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| − | # Servant et al. (2005) CcpN (YqzB), a novel regulator for CcpA-independent catabolite repression of ''Bacillus subtilis'' gluconeogenic genes. Mol. Microbiol. 55: 1435-1451. [http://www.ncbi.nlm.nih.gov/sites/entrez/15720552 PubMed]
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| − | # Licht et al. (2005) Implication of CcpN in the regulation of a novel untranslated RNA (SR1) in ''Bacillus subtilis''. Mol. Microbiol. 58: 189-206. [http://www.ncbi.nlm.nih.gov/sites/entrez/16164558 PubMed]
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| − | # Licht & Brantl (2006) Transcriptional repressor CcpN from ''Bacillus subtilis'' compensates asymmetric contact distribution by cooperative binding. J. Mol. Biol. 364: 434-448. [http://www.ncbi.nlm.nih.gov/sites/entrez/17011578 PubMed]
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| − | # Licht et al. (2008) Identification of ligands affecting the activity of the transcriptional repressor CcpN from ''Bacillus subtilis''. J. Mol. Biol. 380: 17-30. [http://www.ncbi.nlm.nih.gov/sites/entrez/18511073 PubMed]
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| − | # Tännler et al. (2008) CcpN controls central carbon fluxes in ''Bacillus subtilis''. J. Bacteriol. 190: 6178-6187. [http://www.ncbi.nlm.nih.gov/sites/entrez/18586936 PubMed]
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