Difference between revisions of "SalA"

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** number of protein molecules per cell (minimal medium with glucose and ammonium): 841 {{PubMed|24696501}}
 
** number of protein molecules per cell (minimal medium with glucose and ammonium): 841 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3168 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 3168 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1052 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 740 {{PubMed|21395229}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1132 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:'''  
 
* '''Mutant:'''  
 
** 1A919 ( ''salA''::''erm''), {{PubMed|15126467}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A919&Search=1A919 BGSC]
 
** 1A919 ( ''salA''::''erm''), {{PubMed|15126467}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A919&Search=1A919 BGSC]

Revision as of 14:16, 17 April 2014

  • Description: negative regulator of scoC expression

Gene name salA
Synonyms ybaL
Essential no
Product MRP family regulator
Function control of alkaline protease expression
Gene expression levels in SubtiExpress: salA
MW, pI 38 kDa, 5.23
Gene length, protein length 1056 bp, 352 aa
Immediate neighbours cwlD, gerD
Sequences Protein DNA DNA_with_flanks
Genetic context
YbaL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SalA expression.png

























Categories containing this gene/protein

transcription factors and their control, transition state regulators

This gene is a member of the following regulons

SalA regulon

The SalA regulon:

The gene

Basic information

  • Locus tag: BSU01540

Phenotypes of a mutant

increased expression of aprE (due to loss of repression of scoC expression)

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: negative regulation of scoC expression
  • Protein family: Mrp/NBP35 ATP-binding proteins family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation: negative autoregulation
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 841 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 3168 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1052 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 740 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1132 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Mitsuo Ogura, Atsushi Matsuzawa, Hirofumi Yoshikawa, Teruo Tanaka
Bacillus subtilis SalA (YbaL) negatively regulates expression of scoC, which encodes the repressor for the alkaline exoprotease gene, aprE.
J Bacteriol: 2004, 186(10);3056-64
[PubMed:15126467] [WorldCat.org] [DOI] (P p)