Difference between revisions of "SacY"

From SubtiWiki
Jump to: navigation, search
(Extended information on the protein)
Line 87: Line 87:
 
* '''Effectors of protein activity:'''
 
* '''Effectors of protein activity:'''
  
* '''Interactions:''' [[PtsH]]-[[SacY]]
+
* '''[[SubtInteract|Interactions]]:'''
 +
** [[PtsH]]-[[SacY]]
  
* '''Localization:'''
+
* '''[[Localization]]:'''
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 16:09, 10 August 2011

Gene name sacY
Synonyms ipa-13r, sacS
Essential no
Product transcriptional antiterminator
Function regulation of sucrose utilization
Interactions involving this protein in SubtInteract: SacY
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism, Stress
MW, pI 32 kDa, 5.883
Gene length, protein length 840 bp, 280 aa
Immediate neighbours sacX, gspA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacY context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control, RNA binding regulators, phosphoproteins

This gene is a member of the following regulons

SacY regulon

The SacY regulon: sacB-levB-yveA, sacX-sacY

The gene

Basic information

  • Locus tag: BSU38420

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binding to the mRNA of sacB and the sacX-sacY operon, causes transcription antitermination (in presence of sucrose)

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1AUU (RNA-binding domain)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation: induction by sucrose (at high concentration) PubMed
  • Additional information:

Biological materials

  • Mutant: GP425 (cat), available in Stülke lab
  • Expression vector:
    • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag, in pWH844: pGP316, available in Stülke lab
    • for expression, purification of the RNA-binding domain in E. coli with N-terminal His-tag and thrombin cleavage site, in pGP570: pGP573, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

Your additional remarks

References