Difference between revisions of "SacX"
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=== Basic information === | === Basic information === | ||
| − | * '''Locus tag:''' | + | * '''Locus tag:''' BSU38410 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
| Line 81: | Line 81: | ||
* '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P15400 P15400] | * '''Swiss prot entry:''' [http://www.uniprot.org/uniprot/P15400 P15400] | ||
| − | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU38410] |
* '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] | * '''E.C. number:''' [http://www.expasy.org/enzyme/2.7.1.69 2.7.1.69] | ||
Revision as of 12:06, 3 June 2009
- Description: trigger enzyme: sucrose-specific phosphotransferase system, EIIBC component (low affinity)
| Gene name | sacX |
| Synonyms | ipa-14r, sacS |
| Essential | no |
| Product | trigger enzyme: sucrose-specific phosphotransferase system,
EIIBC component (low affinity) |
| Function | sucrose uptake and phosphorylation, control of SacY activity |
| MW, pI | 48 kDa, 9.346 |
| Gene length, protein length | 1377 bp, 459 aa |
| Immediate neighbours | epr, sacY |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU38410
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate (according to Swiss-Prot)
- Protein family: PTS permease, sucrose permease (Scr) family PubMed
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- Structure:
- Swiss prot entry: P15400
- KEGG entry: [3]
- E.C. number: 2.7.1.69
Additional information
Expression and regulation
- Regulation: induction by sucrose (at high concentration)
- Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination)
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
- Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed
- Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
