SacB

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  • Description: levansucrase

Gene name sacB
Synonyms
Essential no
Product levansucrase
Function utilization of sucrose, production of levan
MW, pI 52 kDa, 6.175
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours pbpE, levB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure: 1PT2 (complex with sucrose), 1OYG

Additional information

Expression and regulation

  • Operon:
  • Regulation: induction by sucrose (at high concentration)
  • Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination), in addition activated by DegU-P
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Voigt et al. (2009) Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis. J Mol Microbiol Biotechnol. 16: 53-68 PubMed
  2. Crutz AM, Steinmetz M, Aymerich S, Richter R, Le Coq D (1990) Induction of levansucrase in Bacillus subtilis: an antitermination mechanism negatively controlled by the phosphotransferase system. J Bacteriol 172:1043-1050. PubMed
  3. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed