SacB

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  • Description: levansucrase

Gene name sacB
Synonyms
Essential no
Product levansucrase
Function utilization of sucrose, production of levan
Gene expression levels in SubtiExpress: sacB
Metabolic function and regulation of this protein in SubtiPathways:
sacB
MW, pI 52 kDa, 6.175
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours pbpE, levB
Sequences Protein DNA DNA_with_flanks
Genetic context
SacB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SacB expression.png




























Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

DegU regulon, SacY regulon

The gene

Basic information

  • Locus tag: BSU34450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1PT2 (complex with sucrose), 1OYG
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by sucrose (at high concentration) (SacY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications