sacB
168
levansucrase
locus
BSU_34450
Molecular weight
52.80 kDa
pI
6.18
function
utilization of sucrose, production of levan
product
levansucrase
essential
no
ec
2.4.1.10
synonyms
sacB
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms
This gene is a member of the following regulons
Gene
Coordinates
3,536,012 3,537,433
Phenotypes of a mutant
defective in tomato root colonization in the presence of sucrose [pubmed|33772107]
The protein
Catalyzed reaction/ biological activity
[6)-β-D-fructofuranosyl-(2→](n) α-D-glucopyranoside + sucrose --> [6)-β-D-fructofuranosyl-(2→](n+1) α-D-glucopyranoside + D-glucose (according to UniProt)
Protein family
glycosyl hydrolase 68 family (single member, according to UniProt)
Structure
[PDB|1PT2] (complex with sucrose), [PDB|1OYG]
[AF|P05655]
[wiki|Localization]
secreted (according to Swiss-Prot), extracellular (signal peptide) [Pubmed|18957862]
Biological materials
Mutant
BKE34450 ([gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]::erm trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
BKK34450 ([gene|AAA944BE7F01CE90F7730EECA16F3E4ED77D165A|sacB]::kan trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK34450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGTTCATGTCTCCTTTT, downstream forward: _UP4_TAAAAACGCAAAAGAAAATG
lacZ fusion
pGP564 (in [wiki|pAC7]), a series of RAT mutations in [wiki|pAC7], all available in [wiki|Jörg Stülke]'s lab
References
Reviews
Original Publications
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Time of last update: 2024-04-24 10:54:16
Author of last update: Jstuelk