Difference between revisions of "SacB"

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(Biological materials)
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* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          
* '''lacZ fusion:'''
+
* '''lacZ fusion:''' pGP564 (in [[pAC7]]), a series of RAT mutations in [[pAC7]], all available in [[Stülke]] lab
  
 
* '''GFP fusion:'''
 
* '''GFP fusion:'''

Revision as of 18:32, 29 October 2009

  • Description: levansucrase

Gene name sacB
Synonyms
Essential no
Product levansucrase
Function utilization of sucrose, production of levan
Metabolic function and regulation of this protein in SubtiPathways:
Sugar catabolism, Stress
MW, pI 52 kDa, 6.175
Gene length, protein length 1419 bp, 473 aa
Immediate neighbours pbpE, levB
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
SacB context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU34450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside (according to Swiss-Prot)
  • Protein family: glycosyl hydrolase 68 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: secreted (according to Swiss-Prot), extracellular (signal peptide) PubMed

Database entries

  • Structure: 1PT2 (complex with sucrose), 1OYG
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Operon:
  • Regulation: induction by sucrose (at high concentration)
  • Regulatory mechanism: induction by SacY-dependent RNA switch (transcriptional antitermination), in addition activated by DegU-P
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion: pGP564 (in pAC7), a series of RAT mutations in pAC7, all available in Stülke lab
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References