ruvA

168

Holliday junction DNA helicase, part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] branch migration translocase and the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome

locus: BSU_27740

Molecular weight: 22.44 kDa

pI: 5.38

Protein length: 201 aa

Gene length: 606 bp

function: [wiki|DNA repair/ recombination]

product: Holliday junction DNA helicase

essential: no

synonyms: ruvA

List of homologs in different organisms, belongs to COG0632 (Galperin et al., 2021)

Gene

Coordinates: 2,836,165  2,836,770

Phenotypes of a mutant

• a [gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] [gene|24B1C2A663A5B7C45940272595868F9F05A04A7B|recG] mutant is not viable [pubmed|16020779]
• the inactivations of ''[gene|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal [Pubmed|24770420]
• the inactivations of ''[gene|A3D05FE662CCCFE79B3CB38486206413B84E5D80|recD2]'' and ''[gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]'' are synthetically lethal  [pubmed|28527403]

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The protein

Catalyzed reaction/ biological activity

• inhibits resolution of Holliday junctions by [protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB] [Pubmed|24770420]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• ruvA family (single member, according to UniProt)

Structure

• [PDB|1BDX] (from E. coli, 34% identity) [pubmed|9628481]
• [AF|O05392]

Effectors of protein activity

• [protein|2B091CCEE9E34D659771E39B1FC9050A145048AB|disA] [pubmed|34768753]

[wiki|Interactions]

• part of the [protein|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[protein|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB]-[protein|ED1E2011C7E43A8B7E9FD9A471BC11B9DEA73177|recU] resolvasome [pubmed|24770420]

Biological materials

Mutant

• GP3530 (Δ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]-[gene|8DFD57B73FE5D49BB547E050182A96CE6E8BFF77|ruvB])::kan), available in [wiki|Jörg Stülke]'s lab
• 1A896 (no resistance), [Pubmed|16020779], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A896&Search=1A896 BGSC]
• BKE27740 ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE27740 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG,  downstream forward: _UP4_AAACTATTAAAGTAAGGTGA
• BKK27740 ([gene|7EBC90946E7167A3B0212193873855CAE0EFF7D4|ruvA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK27740 BGSC] and in [wiki|Jörg Stülke]'s lab,  [Pubmed|28189581], upstream reverse: _UP1_CACGCAAATTCACCTCTTTG,  downstream forward: _UP4_AAACTATTAAAGTAAGGTGA

References

Reviews

• 22933559,9442895,38052445

Original Publications

• 16267290,24770420,16020779,9628481,28527403,34768753