Difference between revisions of "RtpA"

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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RtpA RtpA]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=RtpA RtpA]
 
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/pathways/phenyl_tyr_tryp.html Phe, Tyr, Trp]'''
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=rtpA rtpA]'''
 
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|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 4.862   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 5 kDa, 4.862   

Revision as of 10:05, 7 January 2014

  • Description: anti-TRAP

Gene name rtpA
Synonyms yczA
Essential no
Product anti-TRAP
Function regulation of tryptophan biosynthesis
Gene expression levels in SubtiExpress: rtpA
Interactions involving this protein in SubtInteract: RtpA
Metabolic function and regulation of this protein in SubtiPathways:
rtpA
MW, pI 5 kDa, 4.862
Gene length, protein length 159 bp, 53 aa
Immediate neighbours ycbJ, ycbK
Sequences Protein DNA DNA_with_flanks
Genetic context
YczA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RtpA expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, transcription factors and their control

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU02530

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Paul Gollnick, Paul Babitzke, Alfred Antson, Charles Yanofsky
Complexity in regulation of tryptophan biosynthesis in Bacillus subtilis.
Annu Rev Genet: 2005, 39;47-68
[PubMed:16285852] [WorldCat.org] [DOI] (P p)

Original Publications

Joseph R Sachleben, Craig A McElroy, Paul Gollnick, Mark P Foster
Mechanism for pH-dependent gene regulation by amino-terminus-mediated homooligomerization of Bacillus subtilis anti-trp RNA-binding attenuation protein.
Proc Natl Acad Sci U S A: 2010, 107(35);15385-90
[PubMed:20713740] [WorldCat.org] [DOI] (I p)

Yanling Chen, Paul Gollnick
Alanine scanning mutagenesis of anti-TRAP (AT) reveals residues involved in binding to TRAP.
J Mol Biol: 2008, 377(5);1529-43
[PubMed:18334255] [WorldCat.org] [DOI] (I p)

Luis R Cruz-Vera, Ming Gong, Charles Yanofsky
Physiological effects of anti-TRAP protein activity and tRNA(Trp) charging on trp operon expression in Bacillus subtilis.
J Bacteriol: 2008, 190(6);1937-45
[PubMed:18178730] [WorldCat.org] [DOI] (I p)

Mikhail B Shevtsov, Yanling Chen, Paul Gollnick, Alfred A Antson
Crystal structure of Bacillus subtilis anti-TRAP protein, an antagonist of TRAP/RNA interaction.
Proc Natl Acad Sci U S A: 2005, 102(49);17600-5
[PubMed:16306262] [WorldCat.org] [DOI] (P p)

Wen-Jen Yang, Charles Yanofsky
Effects of tryptophan starvation on levels of the trp RNA-binding attenuation protein (TRAP) and anti-TRAP regulatory protein and their influence on trp operon expression in Bacillus subtilis.
J Bacteriol: 2005, 187(6);1884-91
[PubMed:15743934] [WorldCat.org] [DOI] (P p)

Mikhail B Shevtsov, Yanling Chen, Paul Gollnick, Alfred A Antson
Anti-TRAP protein from Bacillus subtilis: crystallization and internal symmetry.
Acta Crystallogr D Biol Crystallogr: 2004, 60(Pt 7);1311-4
[PubMed:15213402] [WorldCat.org] [DOI] (P p)

Doug Snyder, Jeffrey Lary, Yanling Chen, Paul Gollnick, James L Cole
Interaction of the trp RNA-binding attenuation protein (TRAP) with anti-TRAP.
J Mol Biol: 2004, 338(4);669-82
[PubMed:15099736] [WorldCat.org] [DOI] (P p)

Guangnan Chen, Charles Yanofsky
Features of a leader peptide coding region that regulate translation initiation for the anti-TRAP protein of B. subtilis.
Mol Cell: 2004, 13(5);703-11
[PubMed:15023340] [WorldCat.org] [DOI] (P p)

Guangnan Chen, Charles Yanofsky
Tandem transcription and translation regulatory sensing of uncharged tryptophan tRNA.
Science: 2003, 301(5630);211-3
[PubMed:12855807] [WorldCat.org] [DOI] (I p)

Angela Valbuzzi, Charles Yanofsky
Zinc is required for assembly and function of the anti-trp RNA-binding attenuation protein, AT.
J Biol Chem: 2002, 277(50);48574-8
[PubMed:12386162] [WorldCat.org] [DOI] (P p)

Angela Valbuzzi, Paul Gollnick, Paul Babitzke, Charles Yanofsky
The anti-trp RNA-binding attenuation protein (Anti-TRAP), AT, recognizes the tryptophan-activated RNA binding domain of the TRAP regulatory protein.
J Biol Chem: 2002, 277(12);10608-13
[PubMed:11786553] [WorldCat.org] [DOI] (P p)

P Babitzke, P Gollnick
Posttranscription initiation control of tryptophan metabolism in Bacillus subtilis by the trp RNA-binding attenuation protein (TRAP), anti-TRAP, and RNA structure.
J Bacteriol: 2001, 183(20);5795-802
[PubMed:11566976] [WorldCat.org] [DOI] (P p)

A Valbuzzi, C Yanofsky
Inhibition of the B. subtilis regulatory protein TRAP by the TRAP-inhibitory protein, AT.
Science: 2001, 293(5537);2057-9
[PubMed:11557884] [WorldCat.org] [DOI] (P p)

J P Sarsero, E Merino, C Yanofsky
A Bacillus subtilis operon containing genes of unknown function senses tRNATrp charging and regulates expression of the genes of tryptophan biosynthesis.
Proc Natl Acad Sci U S A: 2000, 97(6);2656-61
[PubMed:10706627] [WorldCat.org] [DOI] (P p)