RsmG

• Description: 7-methylguanosine methyltransferase specific for the 16S rRNA, glucose-inhibited division protein
  
  

Gene name	gidB
Synonyms
Essential	no
Product	7-methylguanosine methyltransferase specific for the 16S rRNA
Function	rRNA modification
MW, pI	26 kDa, 6.603
Gene length, protein length	717 bp, 239 aa
Immediate neighbours	noc, gidA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU41000

Phenotypes of a mutant

low-level resistance to streptomycin, increased level of streptomycin-resistant rpsL mutants (200 times higher than in the wild type) PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: methylates the N7 position of nucleotide G535 in 16S rRNA PubMed

• Protein family: RNA methyltransferase rsmG family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1XDZ

• UniProt: P25813

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: thdF-gidA-rsmG-noc

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kenji Nishimura, Shanna K Johansen, Takashi Inaoka, Takeshi Hosaka, Shinji Tokuyama, Yasutaka Tahara, Susumu Okamoto, Fujio Kawamura, Stephen Douthwaite, Kozo Ochi
Identification of the RsmG methyltransferase target as 16S rRNA nucleotide G527 and characterization of Bacillus subtilis rsmG mutants.
J Bacteriol: 2007, 189(16);6068-73
[PubMed:17573471] [WorldCat.org] [DOI] (P p)