Difference between revisions of "RsfA"

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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''rsfA'' {{PubMed|7934828}}
  
* '''Operon:''' ''rsfA''
+
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|16497325,10629188}}, [[SigG]] {{PubMed|10629188}}
 
 
* '''[[Sigma factor]]:''' [[SigF]] {{PubMed|16497325}}
 
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
** expressed early during sporulation in the forespore ([[SigF]]) {{PubMed|16497325,15699190}}
+
** expressed during sporulation in the forespore ([[SigF]]) {{PubMed|16497325,15699190,10629188}}
 
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
 
** repressed by casamino acids [http://www.ncbi.nlm.nih.gov/pubmed/12107147 PubMed]   
  
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=References=
 
=References=
  
<pubmed>16497325,10629188,,12107147, </pubmed>
+
<pubmed>16497325,10629188,7934828 ,12107147,15699190 </pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 20:20, 3 February 2010

  • Description: probable regulator of transcription of SigF-dependent genes

Gene name rsfA
Synonyms ywfN, ipa-92r
Essential no
Product regulator of SigF activity
Function control of expression of SigF-dependent genes
MW, pI 29 kDa, 5.13
Gene length, protein length 774 bp, 258 aa
Immediate neighbours ywzC, ywfM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RsfA context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU37620

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: forespore (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed during sporulation in the forespore (SigF) PubMed
    • repressed by casamino acids PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

L J Wu, J Errington
Identification and characterization of a new prespore-specific regulatory gene, rsfA, of Bacillus subtilis.
J Bacteriol: 2000, 182(2);418-24
[PubMed:10629188] [WorldCat.org] [DOI] (P p)

P Glaser, F Kunst, M Arnaud, M P Coudart, W Gonzales, M F Hullo, M Ionescu, B Lubochinsky, L Marcelino, I Moszer
Bacillus subtilis genome project: cloning and sequencing of the 97 kb region from 325 degrees to 333 degrees.
Mol Microbiol: 1993, 10(2);371-84
[PubMed:7934828] [WorldCat.org] (P p)