RsbU

• Description: protein serine phosphatase, environmental PP2C, dephosphorylates RsbV
  
  

Gene name	rsbU
Synonyms
Essential	no
Product	protein serine phosphatase, environmental PP2C
Function	control of SigB activity
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	38 kDa, 4.906
Gene length, protein length	1005 bp, 335 aa
Immediate neighbours	rsbT, rsbV
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04700

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: O-phospho-L(or D)-serine + H₂O = L(or D)-serine + phosphate (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RsbU-RsbV, RsbS-RsbU, RsbT-RsbU

• Localization:

Database entries

• Structure: 2J6Y

• Swiss prot entry: P40399

• KEGG entry: [3]

• E.C. number: 3.1.3.3

Expression and regulation

• Operon: rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sigB-rsbX PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Bill Haldenwang, San Antonio, USA
• Chet Price, Davis, USA homepage
• Rick Lewis, Newcastle, UK homepage

References

Chien-Cheng Chen, Richard J Lewis, Robin Harris, Michael D Yudkin, Olivier Delumeau
A supramolecular complex in the environmental stress signalling pathway of Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1657-69
[PubMed:12950928] [WorldCat.org] [DOI] (P p)

Sujit Dutta, Richard J Lewis
Crystallization and preliminary crystallographic analysis of the kinase-recruitment domain of the PP2C-type phosphatase RsbU.
Acta Crystallogr D Biol Crystallogr: 2003, 59(Pt 1);191-3
[PubMed:12499568] [WorldCat.org] [DOI] (P p)

U Voelker, A Voelker, W G Haldenwang
The yeast two-hybrid system detects interactions between Bacillus subtilis sigmaB regulators.
J Bacteriol: 1996, 178(23);7020-3
[PubMed:8955331] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed