RpoA

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Gene name rpoA
Synonyms
Essential yes PubMed
Product RNA polymerase alpha subunit
Function transcription
Gene expression levels in SubtiExpress: rpoA
Interactions involving this protein in SubtInteract: RpoA
MW, pI 34 kDa, 4.593
Gene length, protein length 942 bp, 314 aa
Immediate neighbours rpsK, rplQ
Sequences Protein DNA DNA_with_flanks
Genetic context
RpoA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RpoA expression.png















Categories containing this gene/protein

transcription, essential genes, most abundant proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01430

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1) (according to Swiss-Prot)
  • Protein family: RNA polymerase alpha chain family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure: 1Z3E (C-terminal domain, complex with Spx)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • belongs to the 100 most abundant proteins PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 3504 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 11832 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 27116 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 13650 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 25767 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications