Difference between revisions of "RpmI"

From SubtiWiki
Jump to: navigation, search
Line 102: Line 102:
  
 
=== Additional information===
 
=== Additional information===
 +
* the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of [[RbgA]] {{PubMed|23700310}}
  
 
=Expression and regulation=
 
=Expression and regulation=
Line 138: Line 139:
  
 
=References=
 
=References=
<pubmed>19653700 17289755 11948165 21843271 23002217</pubmed>
+
<pubmed>19653700 17289755 11948165 21843271 23002217 23700310</pubmed>
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:37, 12 September 2013

Gene name rpmI
Synonyms
Essential no PubMed
Product ribosomal protein L35
Function translation
Gene expression levels in SubtiExpress: rpmI
Interactions involving this protein in SubtInteract: RpmI
MW, pI 7 kDa, 12.525
Gene length, protein length 198 bp, 66 aa
Immediate neighbours rplT, infC
Sequences Protein DNA DNA_with_flanks
Genetic context
RpmI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RpmI expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

RplT regulon, stringent response

The gene

Basic information

  • Locus tag: BSU28860

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

  • the protein is significantly underrepresented in 45S assembly intermediates that accumulate upon depletion of RbgA PubMed

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Ningning Li, Yuling Chen, Qiang Guo, Yixiao Zhang, Yi Yuan, Chengying Ma, Haiteng Deng, Jianlin Lei, Ning Gao
Cryo-EM structures of the late-stage assembly intermediates of the bacterial 50S ribosomal subunit.
Nucleic Acids Res: 2013, 41(14);7073-83
[PubMed:23700310] [WorldCat.org] [DOI] (I p)

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Patrice Bruscella, Karen Shahbabian, Soumaya Laalami, Harald Putzer
RNase Y is responsible for uncoupling the expression of translation factor IF3 from that of the ribosomal proteins L35 and L20 in Bacillus subtilis.
Mol Microbiol: 2011, 81(6);1526-41
[PubMed:21843271] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Nasslie Choonee, Sergine Even, Lena Zig, Harald Putzer
Ribosomal protein L20 controls expression of the Bacillus subtilis infC operon via a transcription attenuation mechanism.
Nucleic Acids Res: 2007, 35(5);1578-88
[PubMed:17289755] [WorldCat.org] [DOI] (I p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)