Difference between revisions of "RplB"

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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rplB_137311_138144_1 rplB] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=rplB_137311_138144_1 rplB] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
+
* '''[[Sigma factor]]:'''  
 
** [[SigA]] two promoters, about 140bp and 200bp upstream of the start codon {{PubMed|9371452}}
 
** [[SigA]] two promoters, about 140bp and 200bp upstream of the start codon {{PubMed|9371452}}
  
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=References=
 
=References=
'''Additional publications:''' {{PubMed|23002217}}
+
<pubmed> 19653700 9371452 11948165 8635744 10075918 23249812 23002217</pubmed>
<pubmed> 19653700 9371452 11948165 8635744 10075918 23249812 </pubmed>
 
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 10:26, 19 June 2013

Gene name rplB
Synonyms
Essential yes PubMed
Product ribosomal protein L2 (BL2)
Function translation
Gene expression levels in SubtiExpress: rplB
Interactions involving this protein in SubtInteract: RplB
MW, pI 30 kDa, 11.013
Gene length, protein length 831 bp, 277 aa
Immediate neighbours rplW, rpsS
Sequences Protein DNA DNA_with_flanks
Genetic context
RplB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RplB expression.png















Categories containing this gene/protein

translation, essential genes, universally conserved proteins

This gene is a member of the following regulons

stringent response

The gene

Basic information

  • Locus tag: BSU01190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1RL2 (L2 RNA-binding domain, Geobacillus stearothermophilus)
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Josephine R Chandler, Thao T Truong, Patricia M Silva, Mohammad R Seyedsayamdost, Gavin Carr, Matthew Radey, Michael A Jacobs, Elizabeth H Sims, Jon Clardy, E Peter Greenberg
Bactobolin resistance is conferred by mutations in the L2 ribosomal protein.
mBio: 2012, 3(6);
[PubMed:23249812] [WorldCat.org] [DOI] (I e)

Genki Akanuma, Hideaki Nanamiya, Yousuke Natori, Koichi Yano, Shota Suzuki, Shuya Omata, Morio Ishizuka, Yasuhiko Sekine, Fujio Kawamura
Inactivation of ribosomal protein genes in Bacillus subtilis reveals importance of each ribosomal protein for cell proliferation and cell differentiation.
J Bacteriol: 2012, 194(22);6282-91
[PubMed:23002217] [WorldCat.org] [DOI] (I p)

Matthew A Lauber, William E Running, James P Reilly
B. subtilis ribosomal proteins: structural homology and post-translational modifications.
J Proteome Res: 2009, 8(9);4193-206
[PubMed:19653700] [WorldCat.org] [DOI] (P p)

Christine Eymann, Georg Homuth, Christian Scharf, Michael Hecker
Bacillus subtilis functional genomics: global characterization of the stringent response by proteome and transcriptome analysis.
J Bacteriol: 2002, 184(9);2500-20
[PubMed:11948165] [WorldCat.org] [DOI] (P p)

A Nakagawa, T Nakashima, M Taniguchi, H Hosaka, M Kimura, I Tanaka
The three-dimensional structure of the RNA-binding domain of ribosomal protein L2; a protein at the peptidyl transferase center of the ribosome.
EMBO J: 1999, 18(6);1459-67
[PubMed:10075918] [WorldCat.org] [DOI] (P p)

X Li, L Lindahl, Y Sha, J M Zengel
Analysis of the Bacillus subtilis S10 ribosomal protein gene cluster identifies two promoters that may be responsible for transcription of the entire 15-kilobase S10-spc-alpha cluster.
J Bacteriol: 1997, 179(22);7046-54
[PubMed:9371452] [WorldCat.org] [DOI] (P p)

J W Suh, S A Boylan, S H Oh, C W Price
Genetic and transcriptional organization of the Bacillus subtilis spc-alpha region.
Gene: 1996, 169(1);17-23
[PubMed:8635744] [WorldCat.org] [DOI] (P p)