rocF

rocF
168

arginase

locus
BSU_40320
Molecular weight
32.00 kDa
pI
4.93
Protein length
Gene length
function
arginine utilization
product
arginase
essential
no
ec
3.5.3.1
synonyms
rocF, RocF

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0010 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
4,141,711 → 4,142,601
Phenotypes of a mutant
unable to grow with arginine as single source of nitrogen [pubmed|37343703]
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The protein
Catalyzed reaction/ biological activity
H2O + L-arginine --> L-ornithine + urea (according to UniProt)
Protein family
arginase family (with [protein|EA0AA0ECBC4A36A3F01F5876900FE51243D9AD07|speB] and [protein|FBB15E07B7134C57208EA82D5339708385DBC5FB|hutG], according to UniProt)
[wiki|Cofactors]
Mn2+ [pubmed|34160574]
Structure
[PDB|6NFP]
[PDB|6DKT]
[AF|P39138]
Modification
phosphorylated on Ser-68 [Pubmed|20509597]
Kinetic information
Km for arginine: 4.6 mM [pubmed|23625224]
Expression and Regulation
Operons
genes
[gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]
description
[Pubmed|7540694]
regulation
induced in the presence of arginine ([protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]) [Pubmed|7565595]
induced in the presence of arginine, citrulline or ornithine (with ornithine as the molecular inducer) ([protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]) [pubmed|37343703]
regulatory mechanism
[protein|2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A]: repression, [Pubmed|14651647], in [regulon|protein:2C54FE2ADC82FF414D732018C90649D477A925AD|spo0A regulon]
[protein|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]: activation, [Pubmed|7540694], in [regulon|protein:62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC regulon]
[protein|90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY]: repression, [Pubmed|12618455], in [regulon|protein:90ACE0DECA58D3C1A55E135120F7A0C1C920571C|codY regulon]
[protein|BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR]: activation, [Pubmed|7540694], in [regulon|protein:BEE6F16D6A5BBC7FED9E8EB5FA6A94BBF932BBDA|rocR regulon]
sigma factors
[protein|1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL]: sigma factor, [Pubmed|7540694], in [regulon|protein:1118A21CC581B0470EC6E7C178F2523CDCA70F93|sigL regulon]
additional information
expression of the [gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]-[gene|B46A8BCEC56E6252712E30F8BF8DA6B836FDE2F6|rocE]-[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF] operon is increased upon depletion of [gene|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|nusA] (resulting from increased [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC] expression) [http://www.nature.com/articles/nmicrobiol20157 Reference]
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[gene|617FE9E40E8822D58B0446128585CB6CA05A50C6|rocD]→[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]

2024-03-10 09:04:07

Jstuelk

88

2736177DCAE8E1DC5951F68E1BB418B48A3DD8A0

67A3AD2158130CCC732BB0A6D29A52A60C79A4AD

Biological materials
Mutant
GP655, aphA3, available in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
BKE40320 (Δ[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE40320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
BKK40320 (Δ[gene|CD121BBF1DC206D1189ECF8B32456641A55C41D4|rocF]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK40320 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTGTGATTCCACCTCAA,  downstream forward: _UP4_TAATAAGAAAACCCCCGCAC
Expression vectors
pGP3796: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab
pGP3780: expression of Strep-''rocF'' by [wiki|pGP380] in ''B. subtilis'' suitable for [wiki|SPINE], available in [wiki|Jörg Stülke]'s lab
two-hybrid system
''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([wiki|BACTH]), available in [wiki|Jörg Stülke]'s lab
References
Reviews
34160574
Original Publications
12618455,20509597,14651647,12618455,7540694,23625224,10196128,4216455,35787694,35994323,37343703

CD121BBF1DC206D1189ECF8B32456641A55C41D4

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Time of last update: 2024-03-29 15:46:04

Author of last update: Jstuelk