Difference between revisions of "RocF"

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= [[Categories]] containing this gene/protein =
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{{SubtiWiki category|[[utilization of amino acids]]}}
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= This gene is a member of the following [[regulons]] =
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{{SubtiWiki regulon|[[AhrC regulon]]}},
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{{SubtiWiki regulon|[[CodY regulon]]}},
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{{SubtiWiki regulon|[[RocR regulon]]}},
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{{SubtiWiki regulon|[[SigL regulon]]}},
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{{SubtiWiki regulon|[[Spo0A regulon]]}}
  
 
=The gene=
 
=The gene=
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= Categories containing this gene/protein =
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{{SubtiWiki category|[[utilization of amino acids]]}}
 
 
=The protein=
 
=The protein=
  

Revision as of 01:14, 9 December 2010

  • Description: arginase

Gene name rocF
Synonyms
Essential no
Product arginase
Function arginine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 32 kDa, 4.932
Gene length, protein length 888 bp, 296 aa
Immediate neighbours phrG, rocE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocF context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU40320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arginine + H2O = L-ornithine + urea (according to Swiss-Prot)
  • Protein family: arginase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylated on Ser-68 PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2EF5 (from Thermus thermophilus, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by arginine (RocR, AhrC) PubMed
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP655, aphA3, available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References