Difference between revisions of "RocF"

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(Expression and regulation)
(Database entries)
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=== Database entries ===
 
=== Database entries ===
  
* '''Structure:'''
+
* '''Structure:''' [http://www.rcsb.org/pdb/cgi/explore.cgi?pdbId=2EF5 2EF5] (from ''Thermus thermophilus'', 46% identity, 63% similarity)
  
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39138 P39138]
 
* '''UniProt:''' [http://www.uniprot.org/uniprot/P39138 P39138]

Revision as of 18:02, 18 February 2010

  • Description: arginase

Gene name rocF
Synonyms
Essential no
Product arginase
Function arginine utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 32 kDa, 4.932
Gene length, protein length 888 bp, 296 aa
Immediate neighbours phrG, rocE
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocF context.gif
This image was kindly provided by SubtiList







The gene

Basic information

  • Locus tag: BSU40320

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-arginine + H2O = L-ornithine + urea (according to Swiss-Prot)
  • Protein family: arginase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2EF5 (from Thermus thermophilus, 46% identity, 63% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induced by arginine (RocR, AhrC) PubMed
    • repressed under conditions that trigger sporulation (Spo0A) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant: GP655, aphA3, available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Virginie Molle, Yoshiko Nakaura, Robert P Shivers, Hirotake Yamaguchi, Richard Losick, Yasutaro Fujita, Abraham L Sonenshein
Additional targets of the Bacillus subtilis global regulator CodY identified by chromatin immunoprecipitation and genome-wide transcript analysis.
J Bacteriol: 2003, 185(6);1911-22
[PubMed:12618455] [WorldCat.org] [DOI] (P p)

R Gardan, G Rapoport, M Débarbouillé
Expression of the rocDEF operon involved in arginine catabolism in Bacillus subtilis.
J Mol Biol: 1995, 249(5);843-56
[PubMed:7540694] [WorldCat.org] [DOI] (P p)