rocA

168

3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

locus: BSU_37780

Molecular weight: 56.15 kDa

pI: 5.58

Protein length: 515 aa

Gene length: 1548 bp

function: arginine, ornithine and citrulline utilization

product: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

essential: no

ec: 1.5.1.12

synonyms: rocA, ipa-76d

List of homologs in different organisms, belongs to COG1012 (Galperin et al., 2021)

Gene

Coordinates: 3,878,966  3,880,513

Phenotypes of a mutant

• the [gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC] [gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA] double mutant is unable to grow with proline, arginine, citrulline, or ornithine as the single source of nitrogen [pubmed|37343703]

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The protein

Catalyzed reaction/ biological activity

• H₂O + L-glutamate 5-semialdehyde + NAD⁺ --> 2 H⁺ + L-glutamate + NADH (according to UniProt)

Protein family

• [wiki|aldehyde dehydrogenase family] (according to UniProt)

Structure

• [PDB|3RJL] (1-pyrroline-5-carboxylate dehydrogenase from ''Bacillus licheniformis'', 68% identity, 88% similarity)
• [AF|P39634]

Modification

• phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) [Pubmed|17218307]

Paralogous protein(s)

• [protein|762718A15E5256261D79DF60F9106AF0CE2D60C6|iolA], [protein|99F1FAF28FB4817D94E84BD5288FA33124558933|ywdH], [protein|A0BEB92D54799956A4ADE106A1388E5710141069|gabD], [protein|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT], [protein|F3341F205CB939498109D2A54DE842065C488DD5|aldX], [protein|F9CF067A2A8E3B2BACC6A51A0E87E84640349980|aldY], [protein|1ADC55F8E265AF86E29743C671CE1EA1BE7EB41E|gbsA], [protein|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC], [protein|5B66ADB81AB141982F187B1C5E3A9346AA064DE2|ycbD], [protein|67A72A0EABCD807C25D8EAC61C251142B45C174E|dhaS]

Biological materials

Mutant

• BKE37780 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE37780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG,  downstream forward: _UP4_TGATTTGAGAGAAATAAAAT
• BKK37780 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK37780 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATGTAGTCCCCCTCGTG,  downstream forward: _UP4_TGATTTGAGAGAAATAAAAT
• GP3727 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::tet trpC2) available in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
• GP3747 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::aphA3 trpC2) available in in [wiki|Jörg Stülke]'s lab [pubmed|37343703]
• GP3398 ([gene|69838717DC6BB27864D88C282BF5BC7CC558BFD7|rocA]::tet [gene|2042F691CB37B1BAFE2CF3906A9F7F47457C3995|putC]::aphA3 trpC2) available in in [wiki|Jörg Stülke]'s lab [pubmed|37343703]

Expression vectors

• pGP3792: IPTG inducible expression, purification in ''E. coli'' with N-terminal Strep-tag, in [wiki|pGP172], available in [wiki|Jörg Stülke]'s lab

References

• 7565595,12618455,8113162,17218307,20817675,36815589,37343703