Difference between revisions of "RocA"

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(Basic information/ Evolution)
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* '''Protein family:''' RocA subfamily (according to Swiss-Prot)
 
* '''Protein family:''' RocA subfamily (according to Swiss-Prot)
  
* '''Paralogous protein(s):'''
+
* '''Paralogous protein(s):''' [[YcgN]]
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===

Revision as of 12:04, 19 February 2011

  • Description: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

Gene name rocA
Synonyms ipa-76d
Essential no
Product 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase
Function arginine, ornithine and citrulline utilization
Metabolic function and regulation of this protein in SubtiPathways:
Ammonium/ glutamate
MW, pI 56 kDa, 5.58
Gene length, protein length 1545 bp, 515 aa
Immediate neighbours rocB, rocG
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
RocA context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

utilization of amino acids, phosphoproteins

This gene is a member of the following regulons

AhrC regulon, CodY regulon, RocR regulon, SigL regulon

The gene

Basic information

  • Locus tag: BSU37780

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: (S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H (according to Swiss-Prot)
  • Protein family: RocA subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YcgN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) PubMed
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization:

Database entries

  • Structure: 2BJA (with bound NADH from Thermus thermophilus, 43% identity, 59% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • induction by arginine (RocR, AhrC) PubMed
    • repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References