Difference between revisions of "Rnc"
| Line 12: | Line 12: | ||
|style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease III | |style="background:#ABCDEF;" align="center"| '''Product''' || endoribonuclease III | ||
|- | |- | ||
| − | |style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor | + | |style="background:#ABCDEF;" align="center"|'''Function''' || cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[scr]]'') |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 8.076 | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 28 kDa, 8.076 | ||
| Line 57: | Line 57: | ||
=== Basic information/ Evolution === | === Basic information/ Evolution === | ||
| − | * '''Catalyzed reaction/ biological activity:''' | + | * '''Catalyzed reaction/ biological activity:''' cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (''[[scr]]'') |
* '''Protein family:''' | * '''Protein family:''' | ||
Revision as of 14:00, 26 March 2009
- Description: RNase III
| Gene name | rnc |
| Synonyms | rncS |
| Essential | yes |
| Product | endoribonuclease III |
| Function | cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr) |
| MW, pI | 28 kDa, 8.076 |
| Gene length, protein length | 747 bp, 249 aa |
| Immediate neighbours | acpA, smc |
| Gene sequence (+200bp) | Protein sequence |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
essential PubMed
rnc is essential PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor (scr)
- Protein family:
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number: 3.1.26.3
Additional information
Expression and regulation
- Operon:
- Sigma factor:
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Stülke lab
- Antibody:
Labs working on this gene/protein
David Bechhofer, Mount Sinai School, New York, USA Homepage
Your additional remarks
References
- Wang, W. & Bechhofer, D. H. (1997). Bacillus subtilis RNase III gene: cloning, function of the gene in Escherichia coli, and construction of Bacillus subtilis strains with altered rnc loci. J Bacteriol 179, 7379-7385. PubMed
- Herskovitz, M. A. & Bechhofer, D. H. (2000). Endoribonuclease RNase III is essential in Bacillus subtilis. Mol Microbiol 38:1027-1033. PubMed
- Mitra, S. & Bechhofer, D. H. (1994). Substrate specificity of an RNase III-like activity from Bacillus subtilis. J Biol Chem 269:31450-31456. PubMed
- Oguro, A., Kakeshita, H., Nakamura, K., Yamane, K., Wang, W. & Bechhofer, D. H. (1998). Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor. J Biol Chem 273:19542-19547. PubMed
- Yao, S., Blaustein, J. B. & Bechhofer, D. H. (2007). Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site. Nucleic Acids Res. 35:4464-4473. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed
