rlmCD

168

rRNA methyltransferase

locus: BSU_06730

Molecular weight: 51.65 kDa

pI: 5.71

Protein length: 459 aa

Gene length: 1380 bp

function: 23S rRNA maturation

product: rRNA methyltransferase

essential: no

ec: 2.1.1.190

synonyms: rlmCD, yerS, yefA

List of homologs in different organisms, belongs to COG2265 (Galperin et al., 2021)

Gene

Coordinates: 737,603 → 738,982

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The protein

Catalyzed reaction/ biological activity

• methylation of U747 and U1939 in 23S rRNA [Pubmed|21824914]
• S-adenosyl-L-methionine + uridine⁷⁴⁷ in 23S rRNA --> 5-methyluridine⁷⁴⁷ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)
• S-adenosyl-L-methionine + uridine¹⁹³⁹ in 23S rRNA --> 5-methyluridine¹⁹³⁹ in 23S rRNA + H⁺ + S-adenosyl-L-homocysteine (according to UniProt)

Protein family

• [wiki|Methyltransferase superfamily] (according to UniProt)
• [wiki|class I-like SAM-binding methyltransferase superfamily] (according to UniProt)

[wiki|Domains]

• [wiki|TRAM domain] (aa 6-64) (according to UniProt)

[wiki|Cofactors]

• Fe-S cluster [pubmed|29292548]

Structure

• [PDB|2BH2] (from ''E. coli'', 32% identity) [Pubmed|15766524]
• [AF|O31503]

Paralogous protein(s)

• [protein|05392BB2C32B0405799D4F34E5BA55FC936AB943|yfjO]

Biological materials

Mutant

• MGNA-A931 (yefA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/931 NBRP B. subtilis, Japan]
• BKE06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT,  downstream forward: _UP4_TTAAAAGAATAAATAGTCAA
• BKK06730 (Δ[gene|B97ABE3233A27A7C8273ED9A1E688247DD37FD12|rlmCD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06730 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATCTATAATCACCTCTAGT,  downstream forward: _UP4_TTAAAAGAATAAATAGTCAA

References

• 21824914,15766524