RipX

• Description: site-specific integrase/recombinase
  
  

Gene name	ripX
Synonyms	ykqM
Essential	no
Product	site-specific integrase/recombinase
Function	chromosome partitioning
Gene expression levels in SubtiExpress: ripX
MW, pI	33 kDa, 10
Gene length, protein length	888 bp, 296 aa
Immediate neighbours	drm, yqzK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA condensation/ segregation

The gene

Basic information

• Locus tag: BSU23510

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: resolution of chromosome dimers by site specific recombination at the dif site (located close to the terminus region on the chromosome)

• Protein family: XerD subfamily (according to Swiss-Prot)

• Paralogous protein(s): CodV

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)
  • assembles on the chromosome at the dif site throughout the cell cycle PubMed

Database entries

• Structure:

• UniProt: P46352

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ripX PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A793 ( ripX::spec), PubMed, available at BGSC
  • 1A796 ( ripX::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Christine Kaimer, Katrin Schenk, Peter L Graumann
Two DNA translocases synergistically affect chromosome dimer resolution in Bacillus subtilis.
J Bacteriol: 2011, 193(6);1334-40
[PubMed:21239579] [WorldCat.org] [DOI] (I p)

Alexandra E Bloor, Rocky M Cranenburgh
An efficient method of selectable marker gene excision by Xer recombination for gene replacement in bacterial chromosomes.
Appl Environ Microbiol: 2006, 72(4);2520-5
[PubMed:16597952] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, G W Blakely
Identification and characterization of the dif Site from Bacillus subtilis.
J Bacteriol: 2001, 183(3);1058-68
[PubMed:11208805] [WorldCat.org] [DOI] (P p)

S A Sciochetti, P J Piggot, D J Sherratt, G Blakely
The ripX locus of Bacillus subtilis encodes a site-specific recombinase involved in proper chromosome partitioning.
J Bacteriol: 1999, 181(19);6053-62
[PubMed:10498718] [WorldCat.org] [DOI] (P p)