ribA

168

GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase

locus: BSU_23260

Molecular weight: 43.96 kDa

pI: 5.58

Protein length: 398 aa

Gene length: 1197 bp

function: riboflavin biosynthesis

product: GTP cyclohydrolase II/ 3,4-dihydroxy-2-butanone 4-phosphate synthase

essential: no

ec: 3.5.4.25

synonyms: ribA, ribBA

List of homologs in different organisms, belongs to COG0807 (Galperin et al., 2021)

Gene

Coordinates: 2,428,389 → 2,429,585

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The protein

Catalyzed reaction/ biological activity

• D-ribulose 5-phosphate --> (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H⁺ (according to UniProt)
• GTP + 3 H₂O --> 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)-pyrimidine + diphosphate + formate + 2 H⁺ (according to UniProt)

Protein family

• N-terminal part: DHBP synthase family (single member, according to UniProt)
• C-terminal part: GTP cyclohydrolase II family (single member, according to UniProt)

Structure

• [PDB|2BZ0] (from ''E. coli'', 54% identity, 69% similarity to  the C-terminal domain) [Pubmed|16115872]
• [AF|P17620]

[wiki|Localization]

• cytoplasm [pubmed|34474681]

Biological materials

Mutant

• BKE23260 (Δ[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGAAACATGCAAATCTTCC,  downstream forward: _UP4_TAATCACAAATATCACAAAA
• BKK23260 (Δ[gene|F07B7062C850106A95C15978E36DA500F8B089D6|ribA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_ATGAAACATGCAAATCTTCC,  downstream forward: _UP4_TAATCACAAATATCACAAAA

References

• 19583770,12456892,15808508,8159171,7934829,7934830,16115872,23270261,24442413,26280801,26494285,34474681