rhiL

168

[wiki|ABC transporter] (sugar-binding protein) for rhamnose oligosaccharides

locus: BSU_06970

Molecular weight: 46.45 kDa

pI: 4.99

Protein length: 427 aa

Gene length: 1284 bp

function: uptake of rhamnose oligosaccharides

product: rhamnose oligosaccharide [wiki|ABC transporter]

essential: no

synonyms: rhiL, yesO

List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

Gene

Coordinates: 761,662  762,945

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The protein

Protein family

• [wiki|Bacterial solute-binding protein 1 family] (according to UniProt)

Structure

• [PDB|4R6K]
• [PDB|5Z6B] (complexed with rhamnogalacturonan trisaccharide) [pubmed|33122638]
• [PDB|5Z6C] (complexed with rhamnogalacturonan) [pubmed|33122638]
• [AF|O31518]

[wiki|Localization]

• associated to the membrane (via [protein|2DC415DCC2ECC3B46526108638DDB46C28832041|rhiF]-[protein|0B11FACEB5E96D166AC1EA0E32C68FCDAF8327E4|rhiG]) [Pubmed|10092453]

Biological materials

Mutant

• MGNA-A947 (yesO::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/947 NBRP B. subtilis, Japan]
• BKE06970 ([gene|A96A83684E1E6E8705265D3932DA60FF47C23E3B|rhiL]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE06970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA
• BKK06970 ([gene|A96A83684E1E6E8705265D3932DA60FF47C23E3B|rhiL]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK06970 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAAACCAATCCCCCCTTTAA,  downstream forward: _UP4_AATGAGATATTAGAGAGGAA

References

• 10092453,17449691,24391637,33122638,35881471,36815589