Rex

• Description: transcriptional repressor of anaerobically expressed genes involved in anaerobic respiration and fermentation
  
  

Gene name	rex
Synonyms	ydiH
Essential	no
Product	transcriptional repressor
Function	regulation of fermentation and anaerobic respiration
Gene expression levels in SubtiExpress: rex
Metabolic function and regulation of this protein in SubtiPathways: rex
MW, pI	23 kDa, 6.772
Gene length, protein length	645 bp, 215 aa
Immediate neighbours	ydiG, tatAY
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

regulators of electron transport, carbon core metabolism, transcription factors and their control

The gene

Basic information

• Locus tag: BSU05970

Database entries

• BsubCyc: BSU05970

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: transcriptional regulatory rex family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: NADH (indication of low oxygen concentration) releases Rex from DNA PubMed, Rex has a very highh affinity for NADH

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU05970

• Structure: 2VT2, 2VT3

• UniProt: O05521

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: ydiG-rex-tatAY-tatCY PubMed

• Expression browser: rex PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed

Biological materials

• Mutant: LUW292 (erm), GP834 (erm), both available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Claes von Wachenfeldt, Lund University, Sweden Homepage

References

Reviews

Elisabeth Härtig, Dieter Jahn
Regulation of the anaerobic metabolism in Bacillus subtilis.
Adv Microb Physiol: 2012, 61;195-216
[PubMed:23046954] [WorldCat.org] [DOI] (I p)

Original publications

Pierre Nicolas, Ulrike Mäder, Etienne Dervyn, Tatiana Rochat, Aurélie Leduc, Nathalie Pigeonneau, Elena Bidnenko, Elodie Marchadier, Mark Hoebeke, Stéphane Aymerich, Dörte Becher, Paola Bisicchia, Eric Botella, Olivier Delumeau, Geoff Doherty, Emma L Denham, Mark J Fogg, Vincent Fromion, Anne Goelzer, Annette Hansen, Elisabeth Härtig, Colin R Harwood, Georg Homuth, Hanne Jarmer, Matthieu Jules, Edda Klipp, Ludovic Le Chat, François Lecointe, Peter Lewis, Wolfram Liebermeister, Anika March, Ruben A T Mars, Priyanka Nannapaneni, David Noone, Susanne Pohl, Bernd Rinn, Frank Rügheimer, Praveen K Sappa, Franck Samson, Marc Schaffer, Benno Schwikowski, Leif Steil, Jörg Stülke, Thomas Wiegert, Kevin M Devine, Anthony J Wilkinson, Jan Maarten van Dijl, Michael Hecker, Uwe Völker, Philippe Bessières, Philippe Noirot
Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis.
Science: 2012, 335(6072);1103-6
[PubMed:22383849] [WorldCat.org] [DOI] (I p)

Ellen Wang, Teemu P Ikonen, Matti Knaapila, Dmitri Svergun, Derek T Logan, Claes von Wachenfeldt
Small-angle X-ray scattering study of a Rex family repressor: conformational response to NADH and NAD+ binding in solution.
J Mol Biol: 2011, 408(4);670-83
[PubMed:21402078] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ellen Wang, Mikael C Bauer, Annika Rogstam, Sara Linse, Derek T Logan, Claes von Wachenfeldt
Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex.
Mol Microbiol: 2008, 69(2);466-78
[PubMed:18485070] [WorldCat.org] [DOI] (I p)

Smita Gyan, Yoshihiko Shiohira, Ichiro Sato, Michio Takeuchi, Tsutomu Sato
Regulatory loop between redox sensing of the NADH/NAD(+) ratio by Rex (YdiH) and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis.
J Bacteriol: 2006, 188(20);7062-71
[PubMed:17015645] [WorldCat.org] [DOI] (P p)

Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915] [WorldCat.org] [DOI] (P p)

Matthew Schau, Yinghua Chen, F Marion Hulett
Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon.
J Bacteriol: 2004, 186(14);4585-95
[PubMed:15231791] [WorldCat.org] [DOI] (P p)