Difference between revisions of "Rex"
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[[Category:Protein-coding genes]] | [[Category:Protein-coding genes]] | ||
Revision as of 19:29, 16 June 2010
- Description: transcriptional repressor of anaerobically expressed genes involved in anaerobic respiration and fermentation
| Gene name | rex |
| Synonyms | ydiH |
| Essential | no |
| Product | transcriptional repressor |
| Function | regulation of fermentation and anaerobic respiration |
| Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism | |
| MW, pI | 23 kDa, 6.772 |
| Gene length, protein length | 645 bp, 215 aa |
| Immediate neighbours | ydiG, tatAY |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
| |
Contents
The gene
Basic information
- Locus tag: BSU05970
Phenotypes of a mutant
Database entries
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family: transcriptional regulatory rex family (according to Swiss-Prot)
- Paralogous protein(s):
Genes/ operons controlled by Rex
alsS-alsD, cydA-cydB-cydC-cydD, ldh-lctP, ywcJ PubMed
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: NADH (indication of low oxygen concentration) releases Rex from DNA PubMed, Rex has a very highh affinity for NADH
- Interactions:
- Localization: cytoplasm (according to Swiss-Prot)
Database entries
- UniProt: O05521
- KEGG entry: [2]
- E.C. number:
Additional information
Expression and regulation
- Operon:
- Regulation:
- Regulatory mechanism:
- Additional information:
- The mRNA has a long 5' leader region. This may indicate RNA-based regulation PubMed
Biological materials
- Mutant: LUW292 (erm), GP834 (erm), both available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Claes von Wachenfeldt, Lund University, Sweden Homepage
Your additional remarks
References
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Ellen Wang, Mikael C Bauer, Annika Rogstam, Sara Linse, Derek T Logan, Claes von Wachenfeldt
Structure and functional properties of the Bacillus subtilis transcriptional repressor Rex.
Mol Microbiol: 2008, 69(2);466-78
[PubMed:18485070]
[WorldCat.org]
[DOI]
(I p)
Smita Gyan, Yoshihiko Shiohira, Ichiro Sato, Michio Takeuchi, Tsutomu Sato
Regulatory loop between redox sensing of the NADH/NAD(+) ratio by Rex (YdiH) and oxidation of NADH by NADH dehydrogenase Ndh in Bacillus subtilis.
J Bacteriol: 2006, 188(20);7062-71
[PubMed:17015645]
[WorldCat.org]
[DOI]
(P p)
Jonas T Larsson, Annika Rogstam, Claes von Wachenfeldt
Coordinated patterns of cytochrome bd and lactate dehydrogenase expression in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 10);3323-3335
[PubMed:16207915]
[WorldCat.org]
[DOI]
(P p)
Matthew Schau, Yinghua Chen, F Marion Hulett
Bacillus subtilis YdiH is a direct negative regulator of the cydABCD operon.
J Bacteriol: 2004, 186(14);4585-95
[PubMed:15231791]
[WorldCat.org]
[DOI]
(P p)
