resD

168

two-component response regulator ([wiki|OmpR family]), regulation of aerobic and anaerobic respiration, activates expression of target genes in response to oxygen limitation

locus: BSU_23120

Molecular weight: 27.34 kDa

pI: 5.63

Protein length: 240 aa

Gene length: 723 bp

function: regulation of aerobic and anaerobic respiration

product: two-component response regulator ([wiki|OmpR family])

essential: no

synonyms: resD, ypxD

List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 2,417,181  2,417,903

Phenotypes of a mutant

• a ''resD'' point mutation has been isolated upon selction at low pressure [Pubmed|26296725]

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The protein

Protein family

• [wiki|OmpR family] of two-component response regulators

[wiki|Domains]

• [wiki|Response regulatory domain] (aa 8-121) (according to UniProt)

Structure

• [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 37% identity) [pubmed|17942407]
• [AF|P35163]

Modification

• phosphorylated by [protein|EA6790EF30D3DDB9670FE52DFD2C5083AB6A48E1|resE] on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

[wiki|Localization]

• cytoplasm (according to Swiss-Prot)

Biological materials

Mutant

• BKE23120 ([gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE23120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCCCCCTGCTTTC,  downstream forward: _UP4_TATAAATTTGAGGTCGGCGC
• BKK23120 ([gene|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|resD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK23120 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTTTCCCCCTGCTTTC,  downstream forward: _UP4_TATAAATTTGAGGTCGGCGC

labs

• [wiki|Marion Hulett], University of Illinois at Chicago, USA [http://www.uic.edu/depts/bios/faculty/hulett/hulettm.shtml Homepage]

References

Reviews

• 23046954

Original Publications

• 8682783,15576792,16885274,16885456,11325953,10094672,17322317,8631715,9988472,10972837,10972836,9352926,16825793,8631715,11222591,9988472,21091510,22287527,21710567,24214949,27791134,26296725,28439033,17942407,28899391,34898263,36815589