Difference between revisions of "ResB"

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(Expression and regulation)
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* '''[[Sigma factor]]:'''  [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715 PubMed]
 
* '''[[Sigma factor]]:'''  [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715 PubMed]
  
* '''Regulation:'''  
+
* '''Regulation:'''
 
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]
 
** expressed under anaerobic conditions ([[ResD]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]
 
** expressed under conditions of phosphate limitation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9988472 PubMed]
 
** expressed under conditions of phosphate limitation ([[PhoP]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9988472 PubMed]
 +
** repressed by glucose ([[CcpA]]) {{PubMed|16825793}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
 
** [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]
 
** [[ResD]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/8631715,11222591 PubMed]
 
** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9988472 PubMed]
 
** [[PhoP]]: transcription activation [http://www.ncbi.nlm.nih.gov/sites/entrez/9988472 PubMed]
 +
** [[CcpA]]: transcription repression {{PubMed|16825793}}
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=References=
 
=References=
  
<pubmed> 19682263  10844653,8631715,8631715,11222591,9988472, </pubmed>
+
<pubmed> 19682263  10844653,8631715,8631715,11222591,9988472, 16825793</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 11:44, 28 December 2009

  • Description: part of haem translocase, required for cytochrome c synthesis

Gene name resB
Synonyms ypxB
Essential yes PubMed
Product part of the ResB-ResC haem translocase
Function cytochrome c biogenesis
MW, pI 61 kDa, 9.474
Gene length, protein length 1626 bp, 542 aa
Immediate neighbours resC, resA
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ResB context.gif
This image was kindly provided by SubtiList



The gene

Basic information

  • Locus tag: BSU23140

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: haem-binding protein, translocation of haem from the cytoplasmic to the extracytoplasmic site of the membrane PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under anaerobic conditions (ResD) PubMed
    • expressed under conditions of phosphate limitation (PhoP) PubMed
    • repressed by glucose (CcpA) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Umesh Ahuja, Peter Kjelgaard, Benjamin L Schulz, Linda Thöny-Meyer, Lars Hederstedt
Haem-delivery proteins in cytochrome c maturation System II.
Mol Microbiol: 2009, 73(6);1058-71
[PubMed:19682263] [WorldCat.org] [DOI] (I p)

Soo-Keun Choi, Milton H Saier
Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis.
J Mol Microbiol Biotechnol: 2006, 11(1-2);104-10
[PubMed:16825793] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu
Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth.
J Bacteriol: 2001, 183(6);1938-44
[PubMed:11222591] [WorldCat.org] [DOI] (P p)

N E Le Brun, J Bengtsson, L Hederstedt
Genes required for cytochrome c synthesis in Bacillus subtilis.
Mol Microbiol: 2000, 36(3);638-50
[PubMed:10844653] [WorldCat.org] [DOI] (P p)

S M Birkey, W Liu, X Zhang, M F Duggan, F M Hulett
Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD.
Mol Microbiol: 1998, 30(5);943-53
[PubMed:9988472] [WorldCat.org] [DOI] (P p)

G Sun, E Sharkova, R Chesnut, S Birkey, M F Duggan, A Sorokin, P Pujic, S D Ehrlich, F M Hulett
Regulators of aerobic and anaerobic respiration in Bacillus subtilis.
J Bacteriol: 1996, 178(5);1374-85
[PubMed:8631715] [WorldCat.org] [DOI] (P p)